EJG細(xì)胞

細(xì)胞類(lèi)型： 內(nèi)皮細(xì)胞

器官來(lái)源： 腎上腺

是否是腫瘤細(xì)胞： 0

物種來(lái)源： 牛

生長(zhǎng)狀態(tài)： 貼壁生長(zhǎng)

ATCC Number： CRL-8659?

數(shù)量： 大量

相關(guān)**： 正常

細(xì)胞形態(tài)： 內(nèi)皮樣

運(yùn)輸方式： 凍存運(yùn)輸

EJG細(xì)胞組織來(lái)源： capillary endothelium

Designations: EJG

Depositors: The United States of America

Biosafety Level: 2 [Cells contain Bovine Viral Diarrhea Virus (BVDV) ]

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: adherent

Organism: Bos taurus

Morphology: endothelial

Source: Organ: adrenal gland

Tissue: capillary endothelium

Disease: normal

Cell Type: endothelial

Cellular Products: EJG細(xì)胞Factor VIII:C

Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

Comments: The line was derived from collagenase dissociated bovine adrenal tissue.

The cellular morphology is that of capillary endothelium.

The cells form linearly oriented confluent cultures.

Recent tests for bovine viral diarrhea virus (BVDV) indicate that the line is producing both detectable BVDV antigens and infectious BVDV virions (J. Virol Methods 48:211-221, 1994).

Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Temperature: 37.0°C

Subculturing: Medium Renewal: 2 to 3 times per week

Remove medium, rinse flask with fresh 0.25% trypsin, 0.1% EDTA and allow the flask to sit at room temperature until the cells detach (about 5 minutes). EJG細(xì)胞Add fresh medium, aspirate and dispense into new flasks.

Seed new flasks (75 sq. cm.) with 1 X 10 exp4 cells.

Doubling Time: 110 hrs

Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2003

recommended serum:ATCC 30-2020

References: 3866: Ornberg R, et al. Isolation and culture of adrenal medullary endothelial cells producing blood clotting factor VIII:C. US Patent 4,670,394 dated Jun 2 1987

22883: Bolin SR, et al. EJG細(xì)胞Survey of cell lines in the American Type Culture Collection for bovine viral diarrhea virus. J. Virol. Methods 48: 211-221, 1994. PubMed: 7989438