EJ-6-2-Bam-6a細(xì)胞

細(xì)胞形態(tài)： 成纖維樣

生長(zhǎng)狀態(tài)： 貼壁生長(zhǎng)

ATCC Number： CRL-1888?

品系： NIH/Swiss

器官來(lái)源： 胚胎

運(yùn)輸方式： 凍存運(yùn)輸

數(shù)量： 大量

年限： embryo

是否是腫瘤細(xì)胞： 0

EJ-6-2-Bam-6a細(xì)胞物種來(lái)源： 小鼠

Designations: EJ-6-2-Bam-6a

Depositors: RA Weinberg

Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: adherent

Organism: Mus musculus

Morphology: fibroblast

Source: Organ: embryo

Strain: NIH/Swiss

Permits/Forms: EJ-6-2-Bam-6a細(xì)胞In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

Tumorigenic: Yes

Age: embryo

Comments: This line was established by C. Shih et al. in 1981 by transfecting NIH/3T3 cells with Bam H1 digested DNA from EJ-6-2 cells (NIH/3T3 cells transformed with DNA from the human EJ bladder carcinoma EJ).

The cells are morphologically transformed and highly tumorigenic in BALB/c and NSF mice.

Propagation: ATCC complete growth medium: Dulbecco's modified Eagle's medium with 4.5 g/L glucose; 90%; bovine calf serum, 10%

Subculturing: Subcultivation Ratio: A subcultivation ratio of 1:100 is recommended

Medium Renewal: Twice per week

EJ-6-2-Bam-6a細(xì)胞Remove medium, add fresh 0.25% trypsin, 0.03% EDTA, rinse and remove trypsin and let the culture sit at 37C until the cells detach (5 to 10 minutes). Add fresh medium, aspirate and dispense into new flasks. Subculture twice weekly.

References: 22917: Schechter AL, et al. The neu oncogene: an erb-B-related gene encoding a 185,000-Mr tumour antigen. Nature 312: 513-516, 1984. PubMed: 6095109

26163: Shih C, et al. EJ-6-2-Bam-6a細(xì)胞Transforming genes of carcinomas and neuroblastomas introduced into mouse fibroblasts. Nature 290: 261-264, 1981. PubMed: 7207618