356-1細(xì)胞

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產(chǎn)品名稱: 356-1細(xì)胞

產(chǎn)品型號: 356-1

產(chǎn)品展商: HZbscience

產(chǎn)品文檔: 無相關(guān)文檔

簡單介紹

356-1細(xì)胞應(yīng)如何避免細(xì)胞污染，細(xì)胞污染的種類可分成**、酵母菌、霉菌、病毒和霉?jié){菌。主要的污染原因?yàn)闊o菌操作技術(shù)不當(dāng)、操作室環(huán)境不佳、污染之血清和污染之細(xì)胞等。嚴(yán)格之無菌操作技術(shù)、清潔的環(huán)境、與品質(zhì)良好之細(xì)胞來源和培養(yǎng)基配制是減低污染之*好方法。356-1細(xì)胞何時(shí)須更換培養(yǎng)基？視細(xì)胞生長密度而定，或遵照細(xì)胞株基本數(shù)據(jù)上之更換時(shí)間，按時(shí)更換培養(yǎng)基即可。

356-1細(xì)胞 的詳細(xì)介紹

356-1細(xì)胞

細(xì)胞形態(tài)： **樣

**類型： IgG2a; kappa light chain

是否是腫瘤細(xì)胞： 0

物種來源：小鼠

數(shù)量：大量

ATCC Number： HB-181?

生長狀態(tài)：懸浮生長

運(yùn)輸方式：凍存運(yùn)輸

356-1細(xì)胞Designations: 356-1

Depositors: BS Prabhakar

Isotype: IgG2a; kappa light chain

Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: suspension

Organism: Mus musculus (B cell); Mus musculus (myeloma) deposited as mouse (B cell); mouse (myeloma)

Morphology: lymphoblast

Source: Cell Type: hybridoma: B lymphocyte;

Cellular Products: 356-1細(xì)胞immunoglobulin; monoclonal antibody; against coxsackievirus B4 (CB4); also reacts with heart muscle (myocardium); neutralizing

Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

Tumorigenic: Yes

Comments: Animals were immunized with coxsackievirus B4 (JVB strain, ATCC VR 144).

Spleen cells were fused with P3X63Ag8.653 myeloma cells.

The antibody reacts with a moderately conserved epitope on the JVB strain of CB4.

The antibody also reacts with myocardium from several species including mouse, guinea pig, chicken and rabbit.

Neutralizes many human isolates of CB4.

Tested and found negative for ectromelia virus (mousepox).

Propagation: 356-1細(xì)胞ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Subculturing: Medium Renewal: Every 2 to 3 days

Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.

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356-1細(xì)胞