EIAV 12E8.1細(xì)胞

數(shù)量： 大量

細(xì)胞形態(tài)： **樣

**類型： IgG1

ATCC Number： HB-8917?

是否是腫瘤細(xì)胞： 0

物種來源： 小鼠

生長(zhǎng)狀態(tài)： 懸浮生長(zhǎng)

運(yùn)輸方式： 凍存運(yùn)輸

EIAV 12E8.1細(xì)胞Designations: EIAV 12E8.1

Depositors: Fermenta Animal Health Company

Isotype: IgG1

Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: suspension

Organism: Mus musculus (B cell); Mus musculus (myeloma) deposited as mouse (B cell); mouse (myeloma)

Morphology: lymphoblast

Source: Cell Type: hybridoma: B lymphocyte;

Cellular Products: EIAV 12E8.1細(xì)胞immunoglobulin; monoclonal antibody; against equine infectious anemia virus (EIA, EIAV) core antigen (p26)

Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

Comments: Animals were immunized with p26 prepared from the Wyoming strain of EIA virus.

Spleen cells were fused with Sp2/0-Ag14 myeloma cells.

Propagation: EIAV 12E8.1細(xì)胞ATCC complete growth medium: RPMI 1640 medium with 2 mM L-glutamine, 85%; fetal bovine serum, 15%

Subculturing: Medium Renewal: Every 2 to 3 days

Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.

Preservation: DMEM, 70%; fetal bovine serum, 20%; DMSO, 10%

References: 3914: Matsushita T, et al. Method for the detection of equine infectious anemia and other retrovirus infections using a competitive enzyme-linked immunoabsorbent assay and reagents useful in the same. EIAV 12E8.1細(xì)胞US Patent 4,806,467 dated Feb 21 1989