RG2 [D74]細胞

ATCC Number： CRL-2433?

相關(guān)**： 其他**

生長狀態(tài)： 貼壁生長

細胞形態(tài)： 其他

運輸方式： 凍存運輸

年限： fetus, 20 days gestation

器官來源： 大腦

是否是腫瘤細胞： 0

物種來源： 褐鼠

數(shù)量： 大量

Designations: RG2 [D74]

Depositors: RF Barth

RG2 [D74]細胞Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: adherent

Organism: Rattus norvegicus deposited as rat

Morphology: glial

Source: Organ: brain

Disease: differentiated malignant glioma

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Tumorigenic: Yes

Age: fetus, 20 days gestation

Comments: This tumor was produced by Drs. A. Koestner and W. Wechsler at The Ohio State University in 1971.

A pregnant CD Fischer rat on day 20 of gestation was inoculated with a single dose of N-ethyl-N-nitrosourea (ENU) (50 mg/kg b.w.). Tumors were harvested and cloned. RG2 [D74]細胞[38592]

This cell line has been designated RG2 or RG-2 (rat glioma 2) and it is the same as that called D74-RG2 or D74.

This cell line may be used for both in vitro and in vivo studies of a rat brain tumor. It grows well in cell culture and provides a simple, reproducible glioma model when inoculated into the brains of syngeneic rats. [39807]

The biological characteristics of this tumor closely resemble those of human glioblastoma.

The transplantable tumor displays an infiltrative pattern of growth within the brain. [39813]

The RG2 cells are non-immunogenic in syngeneic Fischer rats despite intensive immunization. [38613]

The RG2 and F98 (ATCC CRL-2397) gliomas can be used as rat brain tumor models in experimental neuro-oncology. [39801]

RG2 was submitted to the American Type Culture Collection in March, 1999 at passage 3 from a culture established from a tumor.

Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Temperature: 37.0°C

Subculturing: Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:8 is recommended

Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. RG2 [D74]細胞Allow the flask to sit at room temperature (or at 37C) until the cells detach.

Add fresh culture medium, aspirate and dispense into new culture flasks.

Preservation: Culture medium, 95%; DMSO, 5%

Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2002

recommended serum:ATCC 30-2020

References: 38592: Ko L, et al. Morphological characterization of nitrosourea-induced glioma cell lines and clones. Acta Neuropathol. 51: 23-31, 1980. PubMed: 7435138

38606: Tzeng JJ, et al. Adoptive immunotherapy of a rat glioma using lymphokine-activated killer cells and interleukin 2. Cancer Res. 50: 4338-4343, 1990. PubMed: 2364388

38613: Tzeng JJ, et al. Phenotype and functional activity of tumor-infiltrating lymphocytes isolated from immunogenic and nonimmunogenic rat brain tumors. Cancer Res. 51: 2373-2378, 1991. PubMed: 2015600

39800: Kobayashi N, et al. An improved rat brain-tumor model. J. Neurosurg. 53: 808-815, 1980. PubMed: 7003068

39801: Barth RF. Rat brain tumor models in experimental neuro-oncology: the 9L, C6, T9, F98, RG2 (D74), RT-2 and CNS-1 gliomas. J. Neuro-Oncol. 36: 91-102, 1998. PubMed: 9525831

39803: Swenberg JA, et al. The induction of tumors of the nervous system with intravenous methylnitrosourea. Lab. Invest. 26: 74-85, 1972. PubMed: 4333080

39804: Swenberg JA, et al. RG2 [D74]細胞Quantitative aspects of transplacental tumor induction with ethylnitrosourea in rats. Cancer Res. 32: 2656-2660, 1972. PubMed: 4345400

39807: Aas AT, et al. The RG2 rat glioma model. J. Neuro-Oncol. 23: 175-183, 1995. PubMed: 7673979

39808: Groothuis DR, et al. Permeability of different experimental brain tumor models to horseradish peroxidase. J. Neuropathol. Exp. Neurol. 41: 164-185, 1982. PubMed: 7062086

39809: Groothuis DR, et al. Regional measurements of blood-to-tissue transport in experimental RG-2 rat gliomas. Cancer Res. 43: 3368-3373, 1983. PubMed: 6850641

39811: Rapoport SI. Osmotic opening of the blood-brain barrier. Ann. Neurol. 24: 677-684, 1988. PubMed: 3059990

39812: Ernestus RI, et al. Polyamine metabolism in experimental brain tumors of rat. J. Neurochem. 60: 417-422, 1993. PubMed: 8419528

39813: Ceberg CP, et al. A comparative study on the pharmacokinetics and biodistribution of boronated porphyrin (BOPP) and sulfhydryl boron hydride (BSH) in the RG2 rat glioma model. J. Neurosurg. 83: 86-92, 1995. PubMed: 7782856

39814: Ceberg CP, et al. Enhanced boron uptake in RG 2 rat gliomas by electropermeabilization in vivo--a new possibility in boron neutron capture therapy. Anticancer Drugs 5: 463-466, 1994. PubMed: 7949252

39833: Molnar P, et al. The effects of dexamethasone on experimental brain tumors: I. Transcapillary transport and blood flow in RG-2 rat gliomas. J. Neuro-Oncol. 25: 19-28, 1995. PubMed: 8523086

39835: Devineni D, et al. RG2 [D74]細胞Tissue distribution of methotrexate following administration as a solution and as a magnetic microsphere conjugate in rats bearing brain tumors. J. Neuro-Oncol. 24: 143-152, 1995. PubMed: 7562001

39836: Devineni D, et al. In vivo microdialysis to characterize drug transport in brain tumors: analysis of methotrexate uptake in rat glioma-2 (RG-2)-bearing rats. Cancer Chemother Pharmacol. 38: 499-507, 1996. PubMed: 8823490

39837: Gallo JM, et al. Targeting anticancer drugs to the brain: II. Physiological pharmacokinetic model of oxantrazole following intraarterial administration to rat glioma-2 (RG-2) bearing rats. J. Pharmacokinet. Biopharm. 21: 575-592, 1993. PubMed: 8145132

39839: Tjuvajev J, et al. RG-2 glioma growth attenuation and severe brain edema caused by local production of interleukin-2 and interferon-gamma. Cancer Res. 55: 1902-1910, 1995. PubMed: 7728757