EH17a細胞
生長狀態(tài)： 懸浮生長
運輸方式： 凍存運輸
細胞形態(tài)： **樣
**類型： IgG1
數(shù)量： 大量
ATCC Number： CRL-2209?
是否是腫瘤細胞： 0
物種來源： 小鼠
EH17a細胞Designations: EH17a
Depositors: JD Ernst
Isotype: IgG1
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: suspension
Organism: Mus musculus (B cell); Mus musculus (myeloma) deposited as mouse (B cell); mouse (myeloma)
Morphology: lymphoblast
Source: Cell Type: EH17a細胞hybridoma: B lymphocyte;
Cellular Products: immunoglobulin; monoclonal antibody; against human annexin I
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Tumorigenic: Yes
Comments: Animals were immunized with a mixture of Ca++ dependent phospholipid binding proteins isolated from human polymorphonuclear leukocytes.
Spleen cells were fused with P3X63Ag8.653 myeloma cells.
The antibody reacts with human, bovine and rat annexin I.
It does not inhibit annexin I mediated phospholipid vesicle aggregation.
Propagation: EH17a細胞ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing: Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Preservation: Culture medium, 95%; DMSO, 5%
Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2001
recommended serum:ATCC 30-2020
References: 23407: Ernst JD, et al. Identification of a domain that mediates vesicle aggregation reveals functional diversity of annexin repeats. J. Biol. Chem. 266: 6670-6673, 1991.EH17a細胞 PubMed: 1707872
