SE-1.3細胞

ATCC Number： HB-137?

生長狀態(tài)： 懸浮生長

數(shù)量： 大量

運輸方式： 凍存運輸

是否是腫瘤細胞： 0

物種來源： 小鼠

細胞形態(tài)： **樣

**類型： IgE

SE-1.3細胞Designations: SE-1.3

Depositors: A Nisonoff, S Haba

Isotype: IgE

Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: suspension

Organism: Mus musculus (B cell); Mus musculus (myeloma) deposited as mouse (B cell); mouse (myeloma)

Morphology: lymphoblast

Source: Cell Type: hybridoma: B lymphocyte;

Cellular Products: SE-1.3細胞immunoglobulin; monoclonal antibody; against phenylarsonate

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Comments: Spleen cells from normal A/J were fused with Sp2/0-Ag14 myeloma cells.

Periodic recloning is recommended if the cells are to be maintained in continuous culture.

Tested and found negative for ectromelia virus (mousepox).

Propagation: SE-1.3細胞ATCC complete growth medium: The base medium for this cell line is ATCC Hybri-Care Medium, Catalog No. 46-X. Hybri-Care Medium is supplied as a powder and should be reconstituted in 1 L cell-culture-grade water. To make the complete growth medium, add the following components to the base medium:

fetal bovine serum to a final concentration of 10%

1.5 g/L sodium bicarbonate for use with 5% CO2 in air atmosphere.

Subculturing: Medium Renewal: Every 2 to 3 days

Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 1 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.

Related Products: Recommended medium SE-1.3細胞(without the additional supplements or serum described under ATCC Medium):ATCC 46-X