35.1細胞

數(shù)量： 大量

ATCC Number： HB-222?

運輸方式： 凍存運輸

細胞形態(tài)： **樣

是否是腫瘤細胞： 0

物種來源： 小鼠

**類型： IgG2a

生長狀態(tài)： 懸浮生長

Designations: 35.1

35.1細胞Depositors: PJ Martin

Isotype: IgG2a

Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: suspension

Organism: Mus musculus (B cell); Mus musculus (myeloma) deposited as mouse (B cell); mouse (myeloma)

Morphology: lymphoblast

Source: Cell Type: hybridoma: B lymphocyte;

Cellular Products: immunoglobulin; monoclonal antibody; against human CD2

Permits/Forms: 35.1細胞In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

Comments: Animals were immunized with human T lymphocytes that had been activated with phorbol myristic acid (PMA).

Spleen cells were fused with NSI/1 myeloma cells.

The antibody reacts with the human T cell surface protein Tp50 (CD2).

The antibody inhibits T cell proliferative responses and cytotoxicity, but does not inhibit antibody mediated cellular cytotoxicity.

Propagation: 35.1細胞ATCC complete growth medium: RPMI 1640 medium with 0.05 mM 2-mercaptoethanol and 2 mM L-glutamine, 90%; fetal bovine serum, 10%

Subculturing: Medium Renewal: Every 2 to 3 days

Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 1 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 5 X 10 exp5 cells/ml.

References: 1792: Martin PJ, et al. Identification and functional characterization of two distinct epitopes on the human T cell surface protein Tp50. J. Immunol. 131: 180-185, 1983. 35.1細胞PubMed: 6190907