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產(chǎn)品資料

23A-5-21S細胞

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產(chǎn)品名稱: 23A-5-21S細胞
產(chǎn)品型號: 23A-5-21S
產(chǎn)品展商: HZbscience
產(chǎn)品文檔: 無相關(guān)文檔

簡單介紹

23A-5-21S細胞應(yīng)如何避免細胞污染,細胞污染的種類可分成**、酵母菌、霉菌、病毒和霉?jié){菌。主要的污染原因為無菌操作技術(shù)不當、操作室環(huán)境不佳、污染之血清和污染之細胞等。嚴格之無菌操作技術(shù)、清潔的環(huán)境、與品質(zhì)良好之細胞來源和培養(yǎng)基配制是減低污染之*好方法。23A-5-21S細胞何時須更換培養(yǎng)基?視細胞生長密度而定,或遵照細胞株基本數(shù)據(jù)上之更換時間,按時更換培養(yǎng)基即可。


23A-5-21S細胞  的詳細介紹

23A-5-21S細胞

ATCC Number: HB-36?

運輸方式: 凍存運輸

細胞形態(tài): **樣

**類型: IgM; kappa light chain

數(shù)量: 大量

是否是腫瘤細胞: 0

物種來源: 小鼠

生長狀態(tài): 懸浮生長

Designations: 23A-5-21S

23A-5-21S細胞Depositors: DH Sachs, K Ozato

Isotype: IgM; kappa light chain

Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: suspension

Organism: Mus musculus (B cell); Mus musculus (myeloma) deposited as mouse (B cell); mouse (myeloma)

Morphology: lymphoblast


Source: Cell Type: hybridoma: B lymphocyte;

Cellular Products: immunoglobulin; monoclonal antibody; against H-2 D b; cytotoxic

Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. 23A-5-21S細胞Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

Comments: Animals were immunized with spleen cells from C3H.SW mice.

Spleen cells were fused with Sp2/0-Ag14 myeloma cells.

The antibody cross-reacts with H-2Dd, H-2Da, H-2q and H-2p.

Tested and found negative for ectromelia virus (mousepox).

Propagation: 23A-5-21S細胞ATCC complete growth medium: The base medium for this cell line is ATCC Hybri-Care Medium, Catalog No. 46-X. Hybri-Care Medium is supplied as a powder and should be reconstituted in 1 L cell-culture-grade water. To make the complete growth medium, add the following components to the base medium:

fetal bovine serum to a final concentration of 10%

1.5 g/L sodium bicarbonate for use with 5% CO2 in air atmosphere.

Subculturing: Medium Renewal: Every 2 to 3 days

Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.

Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 46-X

References: 906: Ozato K, Sachs DH.23A-5-21S細胞Monoclonal antibodies to mouse MHC antigens. III. Hybridoma antibodies reacting to antigens of the H-2b haplotype reveal genetic control of isotype expression. J. Immunol. 126: 317-321, 1981. PubMed: 6935293

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