240-13D10細(xì)胞

是否是腫瘤細(xì)胞： 0

物種來源： 小鼠

生長狀態(tài)： 懸浮生長

ATCC Number： CRL-2672?

數(shù)量： 大量

細(xì)胞形態(tài)： **樣

**類型： IgG1 and IgG2b

運輸方式： 凍存運輸

240-13D10細(xì)胞Designations: 240-13D10

Depositors: National Cancer Institute

Isotype: IgG1 and IgG2b

Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: suspension

Organism: Mus musculus (B cell); Mus musculus (myeloma) deposited as mouse (B cell); mouse (myeloma)

Morphology: lymphoblast

Source: Cell Type: 240-13D10細(xì)胞hybridoma: B lymphocyte;

Cellular Products: immunoglobulin; monoclonal antibody; against a c-yes synthetic oncogene peptide

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Comments: Animals were immunized with a synthetic polypeptide with the sequence LMKLCWKKDPDERPTC corresponding to peptides 502 to 517 of the c-yes oncogene (formerly 770 to 793 of the v-yes oncogene).

Spleen cells were fused with Sp2/0 myeloma cells.

In ELISA tests, the antibody reacts strongly with synthetic peptides representing the yes, abl, and ros oncogenes. Weaker activity against src and fgr was also detected.

240-13D10細(xì)胞In immunoblot assays, a protein of approximately 70 kd is detected in a wide variety of cell lines.

The IgG1 reactivity of the antibody with the v-yes peptide (778 to 793) was confirmed by ELISA. IgG1 was strongly positive while IgG2b was only slightly positive.

Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Temperature: 37.0°C

Subculturing: Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density)

Maintain cell density between 2 X 10 exp5 and 1 X 10 exp6 viable cells/ml.

Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 2 X 10 exp5 viable cells/ml.

Preservation: culture medium 95%; DMSO, 5%

Related Products: Recommended medium 240-13D10細(xì)胞(without the additional supplements or serum described under ATCC Medium):ATCC 30-2002

recommended serum:ATCC 30-2020