Renca細(xì)胞
品系： Balb/cCr
年限： 6 weeks
ATCC Number： CRL-2947?
相關(guān)**： 其他**
細(xì)胞類型： 上皮細(xì)胞
是否是腫瘤細(xì)胞： 0
物種來源： 小鼠
數(shù)量： 大量
運(yùn)輸方式： 凍存運(yùn)輸
細(xì)胞形態(tài)： 上皮樣
Renca細(xì)胞生長狀態(tài)： 貼壁生長
器官來源： 腎臟
Designations: Renca
Depositors: R.Wiltrout
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: adherent
Organism: Mus musculus
Morphology: epithelial-like
Source: Organ: kidney
Disease: renal adenocarcinoma
Cell Type: epithelial
Strain: Balb/cCr
Renca細(xì)胞Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Isolation: Isolation date: 1969
Tumorigenic: YES
Age: 6 weeks
Gender: male
Comments: The Renca cell line was derived from a tumor that arose spontaneously as a renal cortical adenocarcinoma in Balb/cCr mice. The pattern of growth of this tumor accurately mimics that of human ***** renal cell carcinoma, particularly with regard to spontaneous metastasis to lung and liver. [PubMed: 4703766, 4057425]The cells do not express transforming growth factor-beta type II receptor (TbetaR-II). [PubMed: 10414746]
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium:
10% fetal bovine serum (final conc.)
non-essential amino acids Renca細(xì)胞(NEAA) (0.1mM extra)
additional sodium pyruvate (1mM extra)
additional L-glutamine (2mM extra)
Temperature: 37.0°C
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Subculturing: Protocol: Volumes used in this protocol are for 75 sq cm flasks; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.
Remove and discard culture medium.
Briefly rinse the cell layer with Ca++/Mg++ free Dulbecco's phosphate-buffered saline (D-PBS) or 0.25% (w/v) Trypsin - 0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor.
Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37C to facilitate dispersal.
Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels. An inoculum of 2 X 10(4) to 4 X 10(4) viable cells/sq. cm is recommended.
Incubate cultures at 37C. Renca細(xì)胞We recommend that you subculture when the culture reaches a cell concentration between 8 X 10(4) and 1.5 X 10(5) cells/sq. cm.
Subcultivation ratio: A subcultivation ratio of 1:4 to 1:10 is recommended.
Medium renewal: Every 2 to 3 days
Preservation: Freeze medium: RPMI-1640 Medium, 77.5%; FBS, 15% FBS; DMSO, 7.5%
Storage temperature liquid nitrogen vapor phase
Doubling Time: approximately 24 hours
Related Products: Recommended medium (without the additional serum described under ATCC Medium): ATCC 30-2001
Recommended serum: ATCC 30-30-2020
0.25% (w/v) Trypsin - 0.53mM EDTA in Hank's BSS (w/o Ca++, Mg ++): ATCC 30-2101
Phosphate-buffered saline: ATCC 30-2200
Cell culture tested DMSO: ATCC 4-X
L-Glutamine solution, 200mM: ATCC 30-2214
Erythrosin B vital stain solution: ATCC 30-2404
References: 16172681: Murphy GP, Hrushesky WJ. A murine renal cell carcinoma. J. Natl. Cancer Inst. 50(4):1013-25, 1973. PubMed: 4703766
16172682: Salup RR, et al. Renca細(xì)胞Role of natural killer activity in development of spontaneous metastases in murine renal cancer. J. Urol. 134(6):1236-41, 1985. PubMed: 4057425
16172683: Engel J, et al. Transforming growth factor-beta type II receptor confers tumor suppressor activity in murine renal carcinoma (renca) cells . Urology. 54(1):164-70, 1999. PubMed: 10414746
