SCC-PSA1細(xì)胞
ATCC Number： CRL-1535?
相關(guān)**： 其他**
是否是腫瘤細(xì)胞： 0
物種來源： 小鼠
數(shù)量： 大量
器官來源： 其他
運(yùn)輸方式： 凍存運(yùn)輸
生長狀態(tài)： 貼壁生長
品系： 129/Sv
Designations: SCC-PSA1
Biosafety Level: 1
Shipped: frozen
SCC-PSA1細(xì)胞Medium & Serum: See Propagation
Growth Properties: adherent
Organism: Mus musculus
Morphology:
Source: Organ: testes
Strain: 129/Sv
Disease: pluripotent teratocarcinoma
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Gender: male
Comments: NOTE - NO LIVE CULTURES CAN BE SENT.
The cells can be maintained in an undifferentiated state by frequent subculture and the use of a fibroblast feeder cell layer (see ATCC CRL-1503).
In the absence of a feeder layer, the cells are encouraged to form aggregates which differentiate forming outer endodermal layers which encapsulate the aggregates.
Aggregates SCC-PSA1細(xì)胞(embryoid bodies) can be encouraged to differentiate further by keeping them in suspension for 5 or more days.
Collect the bodies from a bacteriological dish and plate them in fresh medium in a tissue culture dish without feeder layer or gelatin.
A somewhat variable percentage will attach.
Change the medium every 2 to 3 days.
Migration of the endodermal layer should begin within 24 to 36 hours after plating.
Do not dislodge cells when changing the medium.
The SCC-PSA1 line was isolated from secondary cultures of the OT/5568 transplantable tumor.
Tested and found negative for ectromelia virus (mousepox).
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Temperature: 37.0°C
Subculturing: SCC-PSA1細(xì)胞Medium Renewal: Every 2 to 3 days
Remove medium, add fresh 0.25% trypsin, 0.02% EDTA solution to disperse the cells.
Dilute with fresh medium, centrifuge, and resuspend the cells in fresh medium.
Seed cultures with 8 X 10 exp4 cells/sq cm in flasks with irradiated fibroblast feeder layers (e.g., X-irradiated STO cells, ATCC 56-X).
Subculture every 3 days to maintain in an undifferentiated proliferative state.
Related Products: feeder layer cells:ATCC 56-X
References: 1070: Martin GR, Evans MJ. Differentiation of clonal lines of teratocarcinoma cells: formation of embryoid bodies in vitro. Proc. Natl. Acad. Sci. USA 72: 1441-1445, 1975. PubMed: 1055416
22701: Martin GR, et al. The development of cystic embryoid bodies in vitro from clonal teratocarcinoma stem cells. Dev. Biol. 61: 230-244, 1977. PubMed: 590624
26077: Stevens LC. SCC-PSA1細(xì)胞The development of transplantable teratocarcinomas from intratesticular grafts of pre- and postimplantation mouse embryos. Dev. Biol. 21: 364-382, 1970. PubMed: 5436899
