SCP細(xì)胞
ATCC Number： CRL-1700?
運(yùn)輸方式： 凍存運(yùn)輸
組織來(lái)源： choroid plexus
器官來(lái)源： 大腦
數(shù)量： 大量
是否是腫瘤細(xì)胞： 0
物種來(lái)源： 綿羊
生長(zhǎng)狀態(tài)： 貼壁生長(zhǎng)
Designations: SCP
Depositors: W Siegel, T Booker
SCP細(xì)胞Biosafety Level: 2 [Cells contain Bovine Viral Diarrhea Virus (BVDV) ]
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: adherent
Organism: Ovis aries
Morphology:
Source: Organ: brain
Tissue: choroid plexus
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Virus Susceptibility: Visna virus (strain 1514)
Comments: SCP細(xì)胞The line is positive for bovine viral diarrhea virus (BVD).
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Temperature: 37.0°C
Subculturing: Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended
Medium Renewal: 2 to 3 times per week
Remove medium, rinse with fresh 0.25% trypsin solution, remove trypsin and let the culture sit at room temperature (or at 37C) until the cells detach (about 10 minutes). Add fresh medium, aspirate and dispense into new flasks.
Related Products: SCP細(xì)胞Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2003
recommended serum:ATCC 30-2020
References: 22883: Bolin SR, et al. Survey of cell lines in the American Type Culture Collection for bovine viral diarrhea virus. J. Virol. Methods 48: 211-221, 1994. PubMed: 7989438
32728: Russell DW, Miller AD. Foamy virus vectors. J. Virol. 70: 217-222, 1996. SCP細(xì)胞PubMed: 8523528
