REC-1細胞

細胞類型： **母細胞

是否是腫瘤細胞： 0

物種來源： 人

年限： 57 years

組織來源： lymph node

數(shù)量： 大量

運輸方式： 凍存運輸

細胞形態(tài)： **樣

ATCC Number： CRL-3004?

相關(guān)**： 其他**

REC-1細胞生長狀態(tài)： 懸浮生長

Designations: REC-1

Depositors: E. Campo

Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: suspension

Organism: Homo sapiens

Morphology: lymphoblast

Characterization Data

Source: Tissue: lymph node

Cell type: lymphoblast

Disease: Mantle cell lymphoma (B cell non-Hodgkin's lymphoma)

Permits/Forms: REC-1細胞In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

Isolation: Isolation date: 2003

Applications: Cyclin D1 is overexpressed.

Oncogene: p53

Antigen Expression: CD3-, CD5-, CD10-, CD19+, CD20+, CD23-, FMC7- (verified at ATCC )

DNA Profile (STR): Amelogenin: X,Y

CSF1PO: 10,12

D13S317: 10

D16S539: 11

D5S818: 12,13

D7S820: 10,11

THO1: 9,9.3

TPOX: 8,9

vWA: 17

Cytogenetic Analysis: This is a near-diploid cell line of male origin with a modal chromosome number of 45 and a polyploidy rate of 10%. The karyotype is highly rearranged with most of the cells containing the t(11;14)(q13;q32) common to mantle cell lymphoma. Also, there are approximately 5-6 derivative chromosomes in the karyology that have been described by others. REC-1細胞Note: Cytogenetic information is based on initial seed stock at ATCC .

Age: 57 years

Gender: male

Comments: The human B-cell line Rec-1 was established from a t(11;14)(q13;q32) bearing lymphoma [PubMed: 8142653].

Cyclin D1 is overexpressed. [PubMed: 16434365].

An ongoing level of chromosome rearrangements is observed. [PubMed: 16448697]

Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Subculturing: Protocol: Cultures can be maintained by the addition of fresh medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 X 10(5) viable cells/ml. Maintain cultures at a cell concentration between 3 X 10(5) and 1 X 10(6) cells/ml. Do not allow the cell concentration to exceed 1 x 10(6) cells/ml.

Subcultivation ratio: A subcultivation ratio of 1:3 to 1:6 is recommended.

Interval: Subculture when cell concentration is between 3x10(5) and 1x10(6) cells/ml.

Medium renewal: Add fresh medium every 2 to 3 days (depending on cell density).

Preservation: REC-1細胞Freeze medium: fetal bovine serum, 60%; RPMI-1640 Medium, 30%; DMSO, 10%

Doubling Time: approximately 38 hours

Related Products: Recommended medium (without the additional serum described under ATCC Medium): ATCC 30-2001

Recommended serum: ATCC 30-2020

Cell culture tested DMSO: ATCC 4-X

Erythrosin B vital stain solution: ATCC 30-2404

References: 92895: Salaverria I, et al. Mantle cell lymphoma: from pathology and molecular pathogenesis to new therapeutic perspectives. Haematologica 91: 11-16, 2006. PubMed: 16434365

16172573: Camps, J., et al. Genomic imbalances and patterns of karyotypic variability in mantle-cell lymphoma cell lines. Leuk Res. 30(8):923-934 (2006). PubMed: 16448697

16172765: Rimokh R, et al. Detection of the chromosomal translocation t(11;14) by polymerase chain reaction in mantle cell lymphomas. Blood: 83(7):1871-1875, 1994. PubMed: 8142653

16172766: Pinyol M, et al. Inactivation of RB1 in mantle-cell lymphoma detected by nonsense-mediated mRNA decay pathway inhibition and microarray analysis. Blood: 109(12):5422-5429, 2007. PubMed: 17332242

16172767: Tagawa H, et al. Genome-wide array-based CGH for mantle-cell lymphoma: identification of homozygous deletions of the proapoptotic gene BIM. Oncogene, 1-11, 2004

16172768: Drexler HG, MacLeod RA. Malignant hematopoietic cell lines: in vitro models for the study of mantle cell lymphoma. Leuk. Res. 26: 787-787, 2002. PubMed: 12127550

16172769: REC-1 Product Information, DSMZ (German Collection of Microorganisms and Cell Cultures). Retreived July 09, 2007, from http://www.dsmz.de/index.htm.