RDP 45/20細(xì)胞

ATCC Number： TIB-98?

細(xì)胞形態(tài)： **樣

**類(lèi)型： IgM

是否是腫瘤細(xì)胞： 0

物種來(lái)源： 小鼠

運(yùn)輸方式： 凍存運(yùn)輸

生長(zhǎng)狀態(tài)： 懸浮生長(zhǎng)

數(shù)量： 大量

Designations: RDP 45/20

RDP 45/20細(xì)胞Depositors: WC Raschke

Isotype: IgM

Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: suspension

Organism: Mus musculus (B cell); Mus musculus (myeloma) deposited as mouse (B cell); mouse (myeloma)

Morphology: lymphoblast

Source: Cell Type: hybridoma: B lymphocyte;

Cellular Products: immunoglobulin; RDP 45/20細(xì)胞monoclonal antibody; against the Igh-1b allotypic determinant on mouse IgG2a

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Comments: Animals were immunized with CBPC-101 myeloma protein [IgG2a (Ig-1b)].

Spleen cells were fused with the MPC-11 myeloma cells.

Tested and found negative for ectromelia virus (mousepox).

Propagation: RDP 45/20細(xì)胞ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Subculturing: Medium Renewal: Every 2 to 3 days

Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 x 10 exp5 cells/ml and maintain between 1 x 10 exp5 and 1 x 10 exp6 cells/ml.

References: 2127: DiPauli R, Raschke WC. RDP 45/20細(xì)胞A hybridoma secreting IgM anti-IgIb allotype. Curr. Top. Microbiol. Immunol. 81: 37-39, 1978. PubMed: 567562