E6(2)2細(xì)胞

是否是腫瘤細(xì)胞： 0

物種來源： 小鼠

生長狀態(tài)： 懸浮生長

運(yùn)輸方式： 凍存運(yùn)輸

ATCC Number： HB-8172?

數(shù)量： 大量

細(xì)胞形態(tài)： **樣

**類型： IgG1

Designations: E6(2)2

E6(2)2細(xì)胞Depositors: Research Corporation

Isotype: IgG1

Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: suspension

Organism: Mus musculus (B cell); Mus musculus (myeloma) deposited as mouse (B cell); mouse (myeloma)

Morphology: lymphoblast

Source: Cell Type: hybridoma: B lymphocyte;

Cellular Products: immunoglobulin; E6(2)2細(xì)胞monoclonal antibody; against vitamin B6

Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

Comments: Animals were immunized with phosphopyridoxyl reduced DNA binding proteins.

Spleen cells were fused with P3X63Ag8.653 myeloma cells.

The antibody reacts with B6 vitamers.

It binds to both pyridoxine and pyridoxamine with similar affinities.

E6(2)2細(xì)胞Since the product binds an essential nutrient, the medium must be changed frequently or the cells will die.

Propagation: ATCC complete growth medium: Dulbecco's modified Eagle's medium with 4.5 g/L glucose, and 0.1 mM non-essential amino acids, 70%; NCTC 109 medium, 10%; fetal bovine serum, 20%

Subculturing: Medium Renewal: 3 times per week

Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 1 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.

Preservation: fetal bovine serum, 95%; DMSO, 5%

References: 21902: Cidlowski JA, Viceps-Madore D. E6(2)2細(xì)胞Monoclonal antibodies to vitamin B.sub.6 and immunoassay method. US Patent 4,465,776 dated Aug 14 1984