SCA-9 clone 15細(xì)胞
生長(zhǎng)狀態(tài)： 貼壁生長(zhǎng)
運(yùn)輸方式： 凍存運(yùn)輸
ATCC Number： CRL-1734?
器官來源： 其他
相關(guān)**： 腫瘤
數(shù)量： 大量
是否是腫瘤細(xì)胞： 0
物種來源： 小鼠
細(xì)胞形態(tài)： 成纖維樣
Designations: SCA-9 clone 15
Depositors: T Barka
SCA-9 clone 15細(xì)胞Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: adherent
Organism: Mus musculus
Morphology: fibroblast
Source: Organ: submandibular salivary gland
Disease: carcinoma
Cellular Products: epidermal growth factor (EGF); nerve growth factor (NGF); renin; peptide hydrolase
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Gender: male
Comments: SCA-9 clone 15細(xì)胞This is a soft agar clone of the SCA 124-9 cell line.
The parental line was derived from an undifferentiated carcinoma of the submandibular gland induced by intraglandular injection of 7,12-dimethylbenz[a]anthracene.
The line produces low levels of several factors (e.g., EGF, NGF).
Tested and found negative for ectromelia virus (mousepox).
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Temperature: 37.0°C
Subculturing: Protocol:
Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor.
Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: SCA-9 clone 15細(xì)胞To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37?C to facilitate dispersal.
Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37?C.
Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:4 is recommended
Medium Renewal: 2 to 3 times per week
Preservation: Freeze medium: Complete growth medium, 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor phase
Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2002
recommended serum:ATCC 30-2020
References: 1595: Barka T, et al. SCA-9 clone 15細(xì)胞Epidermal growth factor, renin, and peptidase in cultured tumor cells of submandibular gland origin. Lab. Invest. 42: 656-662, 1980. PubMed: 6993784
