26ic細(xì)胞
是否是腫瘤細(xì)胞： 0
物種來(lái)源： 小鼠
ATCC Number： HB-246?
數(shù)量： 大量
運(yùn)輸方式： 凍存運(yùn)輸
生長(zhǎng)狀態(tài)： 懸浮生長(zhǎng)
細(xì)胞形態(tài)： **樣
**類(lèi)型： IgG2b; kappa light chain
Designations: 26ic
26ic細(xì)胞Depositors: RF Todd
Isotype: IgG2b; kappa light chain
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: suspension
Organism: Mus musculus (B cell); Mus musculus (myeloma) deposited as mouse (B cell); mouse (myeloma)
Morphology: lymphoblast
Source: Cell Type: hybridoma: B lymphocyte;
Cellular Products: 26ic細(xì)胞immunoglobulin; monoclonal antibody; against human CD14
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Comments: Animals were immunized with cultured human peripheral blood monocytes.
Spleen cells were fused with NS-1 myeloma cells.
The antibody reacts with a nonfunctional domain of human CD14.
26ic細(xì)胞The binding of 26ic does not inhibit CD14 mediated activities, and is useful for detecting CD14 expression by immunofluorescence and or immunocytochemical methods.
Propagation: ATCC complete growth medium: Modified Dulbecco's medium, 90%; heat-inactivated gamma globulin free horse serum, 10%
Subculturing: Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 46-X
References: 22487: Todd RF, et al. Structural analysis of differentiation antigens Mo1 and Mo2 on human monocytes. Hybridoma 1: 329-337, 1982. PubMed: 6208133
23506: Wright SD, et al. 26ic細(xì)胞CD14, a receptor for complexes of lipopolysaccharide (LPS) and LPS binding protein. Science 249: 1431-1433, 1990. PubMed: 1698311
