26CB-1細(xì)胞
ATCC Number: CRL-1495?
數(shù)量: 大量
運(yùn)輸方式: 凍存運(yùn)輸
細(xì)胞形態(tài): **樣
細(xì)胞類(lèi)型: **母細(xì)胞
是否是腫瘤細(xì)胞: 0
物種來(lái)源: 其他
生長(zhǎng)狀態(tài): 懸浮生長(zhǎng)
Designations: 26CB-1
26CB-1細(xì)胞Depositors: LA Falk
Biosafety Level: 2 [Cells Contain HERPESVIRUS ]
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: suspension
Organism: Papio hamadryas
Morphology: lymphoblast
Source: Cell Type: lymphoblast
Permits/Forms: 26CB-1細(xì)胞In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Gender: male
Comments: The 26CB-1 cell line was established by co-cultivation of baboon splenic lymphocytes with lethally X-irradiated cells of the 1926 marmoset cell line which carries the HVS transforming virus.
The cells do not produce immunoglobulin.
The cells express a nuclear antigen similar to EBNA and carry Herpesvirus papio.
The cells are E rosette positive.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing: 26CB-1細(xì)胞Medium Renewal: Every 3 to 5 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 viable cells/ml and do not permit the cell density to exceed 2 X 10 exp6 cells/ml.
References: 22853: Falk L, et al. Properties of a baboon lymphotropic herpesvirus related to Epstein-Barr virus. Int. J. Cancer 18: 798-807, 1976. PubMed: 186420
22856: Falk LA, et al. Transformation of lymphocytes by Herpesvirus papio. Int. J. Cancer 20: 219-226, 1977. PubMed: 197025
22857: Ohno S, et al. Detection of a nuclear, EBNA-type antigen in apparently EBNA-negative Herpesvirus papio (HVP)-transformed lymphoid lines by the acid-fixed nuclear binding technique. Int. J. Cancer 20: 941-946, 1977. PubMed: 73523
23145: Klein G, et al. Antigen-inducing ability of herpesvirus papio in human and baboon lymphoma lines, compared to Epstein-Barr virus. Intervirology 10: 153-164, 1978.26CB-1細(xì)胞 PubMed: 210135