RBL-1細(xì)胞
生長(zhǎng)狀態(tài)： 懸浮生長(zhǎng)
運(yùn)輸方式： 凍存運(yùn)輸
品系： Wistar
數(shù)量： 大量
細(xì)胞類(lèi)型： 其他細(xì)胞類(lèi)型
是否是腫瘤細(xì)胞： 0
物種來(lái)源： 大鼠
ATCC Number： CRL-1378?
相關(guān)**： 白血病
RBL-1細(xì)胞細(xì)胞形態(tài)： **樣
器官來(lái)源： 外周血
Designations: RBL-1
Depositors: H Metzger, C Isersky
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: suspension
Organism: Rattus norvegicus deposited as Rattus sp.
Morphology:RBL-1細(xì)胞 lymphoblast
Source: Organ: peripheral blood
Strain: Wistar
Disease: leukemia
Cell Type: basophil; chemically induced
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Applications: transfection host
Receptors: RBL-1細(xì)胞FcERI (Fc of IgE)
Comments: The line exhibits various characteristics of basophil differentiation including surface receptors for IgE. It was shown to not release histamine by an IgE mediated system. PubMed: 6166481 [22475]
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Temperature: 37.0°C
Subculturing: Protocol: Cultures can be maintained by the addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 viable cells/ml and maintain between 1 X 10 exp5 cells/ml and 1 X 10 exp6 /ml..
Medium Renewal: Add medium as cell density increases
Preservation: Freeze medium: Complete growth medium 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor phase
Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2003
recommended serum:ATCC 30-2020
References: 1232: Kulczycki A Jr., et al. RBL-1細(xì)胞The interaction of IgE with rat basophilic leukemia cells. I. Evidence for specific binding of IgE. J. Exp. Med. 139: 600-616, 1974. PubMed: 4812630
22475: Barsumian EL, et al. IgE-induced histamine release from rat basophilic leukemia cell lines: isolation of releasing and nonreleasing clones. Eur. J. Immunol. 11: 317-323, 1981. PubMed: 6166481
22638: Eccleston E, et al. Basophilic leukaemia in the albino rat and a demonstration of the basopoietin. Nat. New Biol. 244: 73-76, 1973.
