E. Derm (NBL-6)細(xì)胞

生長(zhǎng)狀態(tài)： 貼壁生長(zhǎng)

年限： 4 years

器官來(lái)源： 皮膚

ATCC Number： CCL-57?

運(yùn)輸方式： 凍存運(yùn)輸

細(xì)胞形態(tài)： 成纖維樣

數(shù)量： 大量

品系： quarterhorse

組織來(lái)源： dermis

E. Derm (NBL-6)細(xì)胞是否是腫瘤細(xì)胞： 0

物種來(lái)源： 其他

Designations: E. Derm (NBL-6)

Depositors: AJ Kniazeff, WA Nelson-Rees, NB Darby

Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: adherent

Organism: Equus caballus

Morphology: fibroblast

Source: Organ: skin

Strain: E. Derm (NBL-6)細(xì)胞quarterhorse

Tissue: dermis

Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

Isolation: Isolation date: September, 1965

Virus Resistance: adenovirus 5; coxsackievirus A9, B5; poliovirus 2

Cytogenetic Analysis: model number = 64; range = 52 to 145. The donor's sex (female) is confirmed chromosomally. Although the chromosome numbers vary widely, 55% of the cells have a chromosome number within + or - 1 of the diploid and modal number (64).

Age: 4 years

Gender: female

Comments: Cells appear to senesce after 40 passages.

Propagation: E. Derm (NBL-6)細(xì)胞ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Temperature: 37.0°C

Subculturing: Protocol: Remove medium, and rinse with 0.25% trypsin, 0.53 mM EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach. Add fresh culture medium, aspirate and dispense into new culture flasks.

Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:6 is recommended

Medium Renewal: Two to three times weekly

Preservation: Freeze medium: Complete growth medium, 95%; DMSO, 5%

Storage temperature: liquid nitrogen vapor temperature

Related Products: E. Derm (NBL-6)細(xì)胞Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2003

recommended serum:ATCC 30-2020