SC細(xì)胞

器官來(lái)源： 外周血

數(shù)量： 大量

細(xì)胞形態(tài)： 單核細(xì)胞/巨噬細(xì)胞

生長(zhǎng)狀態(tài)： 懸浮生長(zhǎng)

ATCC Number： CRL-9855?

細(xì)胞類(lèi)型： 其他細(xì)胞類(lèi)型

是否是腫瘤細(xì)胞： 0

物種來(lái)源： 人

SC細(xì)胞運(yùn)輸方式： 凍存運(yùn)輸

Designations: SC

Depositors: E.I. du Pont de Nemours & Co.

Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: suspension

Organism: Homo sapiens

Morphology: monocyte/macrophage

Source: Organ: peripheral blood

Cell Type: SC細(xì)胞monocyte/macrophage

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Comments: The SC cell line was established from human peripheral blood mononuclear cells.

The cells grow as single cells in suspension, and have a granular appearance.

Propagation: ATCC complete growth medium: Iscove's modified Dulbecco's medium with 4 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate and supplemented with 0.05 mM 2-mercaptoethanol, 0.1 mM hypoxanthine and 0.016 mM thymidine, 90%; fetal bovine serum, 10%

Atmosphere: air, 95%; carbon dioxide (CO2), 5%

Temperature: 37.0°C

Subculturing: Protocol: Cultures can be maintained by addition or replacement of fresh medium.

Interval: SC細(xì)胞Maintain the cell concentration between 2 X 10(5) and 1 X 10 (6) cells/ml)

Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density)

Preservation: Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO

Storage temperature: liquid nitrogen vapor phase

Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2005

recommended serum:ATCC 30-2020

References: 26300: Collins GW, Largen MT. SC細(xì)胞Continuous mammalian cell lines having monocyte/macrophage characteristics and their establishment in vitro. US Patent 5,447,861 dated Sep 5 1995