RAW 8.1細胞

生長狀態(tài)： 懸浮生長

品系： BALB/c

細胞類型： B**細胞

是否是腫瘤細胞： 0

物種來源： 小鼠

細胞形態(tài)： **樣

ATCC Number： TIB-50?

相關**： **瘤

RAW 8.1細胞數(shù)量： 大量

運輸方式： 凍存運輸

器官來源： 脾

Designations: RAW 8.1

Depositors: P Ralph

Biosafety Level: 2

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: suspension

Organism: Mus musculus

Morphology: lymphoblast

Source: Organ: RAW 8.1細胞spleen

Strain: BALB/c

Disease: lymphoma

Cell Type: B lymphocyte;

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Comments: RAW 8.1 was established from a tumor induced by Abelson murine leukemia virus.

Growth of this line is inhibited by 5 mcg/ml LPS.

Tested and found negative for ectromelia virus (mousepox).

Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. RAW 8.1細胞To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Subculturing: Medium Renewal: Every 2 to 3 days

Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.

References: 1137: Ralph P, Nakoinz I. Lipopolysaccharides inhibit lymphosarcoma cells of bone marrow origin. Nature 249: 49-51, 1974. PubMed: 4208429

2104: Ralph P, et al. Lymphosarcoma cell growth is selectively inhibited by B lymphocyte mitogens: LPS, dextran sulfate and PPD. Biochem. Biophys. Res. Commun. 61: 1268-1275, 1974. RAW 8.1細胞PubMed: 4616699