RAW 309F.1.1細(xì)胞

生長狀態(tài)： 懸浮生長

數(shù)量： 大量

細(xì)胞形態(tài)： **樣

運(yùn)輸方式： 凍存運(yùn)輸

是否是腫瘤細(xì)胞： 0

物種來源： 小鼠

器官來源： 脾

ATCC Number： TIB-51?

相關(guān)**： **瘤

RAW 309F.1.1細(xì)胞Designations: RAW 309F.1.1

Depositors: WC Raschke

Biosafety Level: 2

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: suspension

Organism: Mus musculus

Morphology: lymphoblast

Source: Organ: spleen

Disease: lymphoma

Cellular Products: Abelson murine leukemia virus (infectious)

Permits/Forms: RAW 309F.1.1細(xì)胞In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

Antigen Expression: H-2d; H-2b

Comments: The cells are negative for surface immunoglobulin (sIg-), Thy-1.2 (Thy-1.2-) and Ia (Ia-).

The cells do not pinocytose neutral red, phagocytose zymosan or latex beads and do not secrete lysozyme.

The line was derived from the RAW 309 tumor (see also ATCC TIB-69).

RAW 309F.1.1細(xì)胞Tested and found negative for ectromelia virus (mousepox).

Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Subculturing: Medium Renewal: Every 2 to 3 days

Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.

References: 1207: Raschke WC, et al. RAW 309F.1.1細(xì)胞Functional macrophage cell lines transformed by Abelson leukemia virus. Cell 15: 261-267, 1978. PubMed: 212198