UV5 (UV sensitive mutant of CHO)細(xì)胞

是否是腫瘤細(xì)胞： 0

物種來(lái)源： 倉(cāng)鼠

細(xì)胞形態(tài)： 上皮樣

ATCC Number： CRL-1865?

運(yùn)輸方式： 凍存運(yùn)輸

生長(zhǎng)狀態(tài)： 單層、懸浮

數(shù)量： 大量

器官來(lái)源： 卵巢

Designations: UV5 (UV sensitive mutant of CHO)

UV5 (UV sensitive mutant of CHO)細(xì)胞Depositors: LH Thompson

Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: monolayer and suspension

Organism: Cricetulus griseus

Morphology: epithelial-like

Source: Organ: ovary

Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

Gender: female

Comments: UV5 (UV sensitive mutant of CHO)細(xì)胞This line is a derivative of the CHO-K1 cell line (see ATCC CCL-61).

UV5 is a UV sensitive line derived from AA8 (see ATCC CRL-1859).

The line is defective in nucleotide excision repair, is sensitive to bulky adduct mutagens and belongs to excision repair complementation group 2.

Propagation: ATCC complete growth medium: Alpha minimum essential medium without ribonucleosides and deoxyribonucleosides, 90%; fetal bovine serum, 10%

Subculturing: Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:12 is recommended

Medium Renewal: 2 to 3 times per week

To subculture attached cells, remove the medium and rinse the cells with fresh 0.25% trypsin, 0.03% EDTA.

Let the culture sit at 37C until the cells detach (about 5 minutes).

Add fresh medium, aspirate and transfer to new flasks.

The suspended cells are viable and can be used to start new cultures.

Doubling Time: 13 hrs

References: 1767: Thompson LH, et al. Repair of DNA adducts in asynchronous CHO cells and the role of repair in cell killing and mutation induction in synchronous cells treated with 7-bromomethylbenz[a]anthracene. Somatic Cell Mol. Genet. 10: 183-194, 1984. PubMed: 6584989

1768: Thompson LH, et al. UV5 (UV sensitive mutant of CHO)細(xì)胞Genetic diversity of UV-sensitive DNA repair mutants of Chinese hamster ovary cells. Proc. Natl. Acad. Sci. USA 78: 3734-3737, 1981. PubMed: 6943579

1769: Hoy CA, et al. Defective DNA cross-link removal in Chinese hamster cell mutants hypersensitive to bifunctional alkylating agents. Cancer Res. 45: 1737-1743, 1985. PubMed: 3919945

1770: Busch D, et al. Summary of complementation groups of UV-sensitive CHO cell mutants isolated by large-scale screening. Mutagenesis 4: 349-354, 1989. PubMed: 2687628

32479: Bessho T, et al. Initiation of DNA interstrand cross-link repair in humans: the nucleotide excision repair system makes dual incisions 5" to the cross-linked base and removes a 22- to 28-nucleotide-long damage-free strand. Mol. Cell. Biol. 17: 6822-6830, 1997. PubMed: 9372913

32924: Reardon JT, et al. Isolation and characterization of two human transcription factor IIH (TFIIH)-related complexes: ERCC2/CAKand TFIIH. Proc. Natl. Acad. Sci. USA 93: 6482-6487, 1996. PubMed: 8692841

58395: Thompson LH, et al. Hypersensitivity to mutation and sister-chromatid-exchange induction in CHO cell mutants defective in incising DNA containing UV lesions. Somatic Cell Genet. 8: 759-773, 1982. PubMed: 7163954

58396: Thompson LH, et al. UV5 (UV sensitive mutant of CHO)細(xì)胞A screening method for isolating DNA repair-deficient mutants of CHO cells. Somatic Cell Genet. 6: 391-405, 1980. PubMed: 7404270