26-8-16S細胞

ATCC Number： HB-42?

生長狀態(tài)： 懸浮生長

運輸方式： 凍存運輸

細胞形態(tài)： **樣

**類型： IgM

是否是腫瘤細胞： 0

物種來源： 小鼠

數量： 大量

Designations: 26-8-16S

26-8-16S細胞Depositors: DH Sachs, K Ozato

Isotype: IgM

Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: suspension

Organism: Mus musculus (B cell); Mus musculus (myeloma) deposited as mouse (B cell); mouse (myeloma)

Morphology: lymphoblast

Source: Cell Type: hybridoma: B lymphocyte;

Cellular Products: immunoglobulin; monoclonal antibody; against I-A k; cytotoxic

Permits/Forms: 26-8-16S細胞In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

Comments: Animals were immunized with spleen cells from A.TL mice.

Spleen cells were fused with Sp2/0-Ag14 myeloma cells.

Tested and found negative for ectromelia virus (mousepox).

Propagation: 26-8-16S細胞ATCC complete growth medium: The base medium for this cell line is ATCC Hybri-Care Medium, Catalog No. 46-X. Hybri-Care Medium is supplied as a powder and should be reconstituted in 1 L cell-culture-grade water. To make the complete growth medium, add the following components to the base medium:

fetal bovine serum to a final concentration of 10%

1.5 g/L sodium bicarbonate for use with 5% CO2 in air atmosphere.

Subculturing: Medium Renewal: Every 2 to 3 days

Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.

Related Products: Recommended medium 26-8-16S細胞(without the additional supplements or serum described under ATCC Medium):ATCC 46-X