UV20 (UV sensitive mutant of CHO)細胞

器官來源： 卵巢

ATCC Number： CRL-1862?

細胞形態(tài)： 成纖維樣

數(shù)量： 大量

是否是腫瘤細胞： 0

物種來源： 倉鼠

生長狀態(tài)： 混合型生長

運輸方式： 凍存運輸

Designations: UV20 (UV sensitive mutant of CHO)

Depositors: LH Thompson

UV20 (UV sensitive mutant of CHO)細胞Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: mixed, adherent and suspension

Organism: Cricetulus griseus

Morphology: fibroblast

Source: Organ: ovary

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Gender: female

Comments: This line is a derivative of the CHO-K1 cell line (see ATCC CCL-61).

UV20 is a UV sensitive line derived from AA8 (see ATCC CRL-1859).

UV20 (UV sensitive mutant of CHO)細胞The line is defective in nucleotide excision repair, is sensitive to bulky adduct mutagens and belongs to excision repair cross complementation group 1.

Both UV20 and UV41 (see ATCC CRL-1860) are extremely sensitive to DNA cross linking agents.

Propagation: ATCC complete growth medium: Alpha minimum essential medium without ribonucleosides and deoxyribonucleosides, 90%; fetal bovine serum, 10%

Temperature: 37.0°C

Subculturing: Protocol: To subculture attached cells, remove the medium and rinse the cells with fresh 0.25% trypsin, 0.03% EDTA. Let the culture sit at 37C until the cells detach (about 5 minutes). The suspended cells are viable and can be used to start new cultures.

Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:12 is recommended

Medium Renewal: 2 to 3 times per week

Doubling Time: 16 hrs

Related Products: recommended serum:ATCC 30-2020

parental cell line:ATCC CRL-1859

References: 1767: UV20 (UV sensitive mutant of CHO)細胞Thompson LH, et al. Repair of DNA adducts in asynchronous CHO cells and the role of repair in cell killing and mutation induction in synchronous cells treated with 7-bromomethylbenz[a]anthracene. Somatic Cell Mol. Genet. 10: 183-194, 1984. PubMed: 6584989

1768: Thompson LH, et al. Genetic diversity of UV-sensitive DNA repair mutants of Chinese hamster ovary cells. Proc. Natl. Acad. Sci. USA 78: 3734-3737, 1981. PubMed: 6943579

1769: Hoy CA, et al. Defective DNA cross-link removal in Chinese hamster cell mutants hypersensitive to bifunctional alkylating agents. Cancer Res. 45: 1737-1743, 1985. PubMed: 3919945

1770: Busch D, et al. Summary of complementation groups of UV-sensitive CHO cell mutants isolated by large-scale screening. Mutagenesis 4: 349-354, 1989. PubMed: 2687628

32479: Bessho T, et al. Initiation of DNA interstrand cross-link repair in humans: the nucleotide excision repair system makes dual incisions 5" to the cross-linked base and removes a 22- to 28-nucleotide-long damage-free strand. Mol. Cell. Biol. 17: 6822-6830, 1997. PubMed: 9372913

58395: Thompson LH, et al. Hypersensitivity to mutation and sister-chromatid-exchange induction in CHO cell mutants defective in incising DNA containing UV lesions. Somatic Cell Genet. 8: 759-773, 1982. PubMed: 7163954

58396: Thompson LH, et al. UV20 (UV sensitive mutant of CHO)細胞A screening method for isolating DNA repair-deficient mutants of CHO cells. Somatic Cell Genet. 6: 391-405, 1980. PubMed: 7404270