SA22細胞
是否是腫瘤細胞： 0
物種來源： 小鼠
數(shù)量： 大量
細胞形態(tài)： **樣
**類型： IgG1
生長狀態(tài)： 懸浮生長
ATCC Number： CRL-2052?
運輸方式： 凍存運輸
Designations: SA22
SA22細胞Depositors: F Blecha
Isotype: IgG1
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: suspension
Organism: Mus musculus (B cell); Mus musculus (myeloma) deposited as mouse (B cell); mouse (myeloma)
Morphology: lymphoblast
Source: Cell Type: hybridoma: B lymphocyte;
Cellular Products: immunoglobulin; monoclonal antibody; against bovine recombinant interleukin 1 beta (rboIL-1 beta)
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. SA22細胞Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Tumorigenic: Yes
Comments: Animals were immunized with purified rboIL-1 beta.
Spleen cells were fused with P3X63Ag8.653 myeloma cells.
The antibody reacts with rboIL-1 beta and cross-reacts with its human analog (rHuIL-1 beta).
It does not react with other related monokines tested (ie.g., human IL-6 and TNF alpha).
The antibodies neutralizes IL-1 activity (both macrophage derived IL-1 and recombinant IL-1 beta) in a thymocyte costimulation assay.
SA22細胞Propagation: ATCC complete growth medium: Dulbecco's modified Eagle's medium with 4.5 g/L glucose, 1 mM non-essential amino acids, 0.1 mM hypoxanthine, 0.032 mM thymidine and BME vitamins, 70%; hybridoma cloning factor (Origen), 10%; fetal bovine serum, 20%
Subculturing: Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
References: 22352: Reddy DN, et al. Isolation and characterization of monoclonal antibodies to recombinant bovine interleukin-1 beta. Vet. Immunol. Immunopathol. 36: 17-29, 1993. SA22細胞PubMed: 7680508
