SaI [Sa I, Sarcoma I]細(xì)胞

生長(zhǎng)狀態(tài)： 懸浮生長(zhǎng)

數(shù)量： 大量

運(yùn)輸方式： 凍存運(yùn)輸

ATCC Number： CRL-2543?

相關(guān)**： 其他**

細(xì)胞形態(tài)： **樣

細(xì)胞類(lèi)型： 其他細(xì)胞類(lèi)型

是否是腫瘤細(xì)胞： 0

物種來(lái)源： 小鼠

SaI [Sa I, Sarcoma I]細(xì)胞Designations: SaI [Sa I, Sarcoma I]

Depositors: S Ostrand-Rosenberg

Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: suspension

Organism: Mus musculus deposited as mouse

Morphology: lymphoblast

Source: Disease: fibrosarcoma, malignant

Cell Type: dibenzanthracene induced

Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. SaI [Sa I, Sarcoma I]細(xì)胞Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

Tumorigenic: Yes

Antigen Expression: MHC class I +; MHC class II - [49807]

H-2a

Comments: The Sarcoma I tumor originated in 1947 in a mouse that had been treated with dibenzanthracene [53560]

The tumor grows progressively in the A/Jax subline of the Strong A mouse and kills 100% of the animals. In contrast, mice of the resistant C57BL/6K strain reject the tumor in about 100% of the cases. [53559]

The SaI variant (ATCC CRL-2543) grows as an ascites tumor when inoculated intraperitoneally, while the SaI/N variant grows as a solid tumor when inoculated subcutaneously [49807]

The cell line can be used as a model for testing immunotherapy protocols. It can be used in immunology and cancer studies. The cells may be used for transfection studies.

Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Iscove's Modified Dulbecco's Medium, Catalog No. 30-2005. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

SaI [Sa I, Sarcoma I]細(xì)胞Atmosphere: air, 95%; carbon dioxide (CO2), 5%

Temperature: 37.0°C

Subculturing: Protocol: Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 5 X 10(5) viable cells/ml.

Interval: Maintain cell density between 5 X 10(5) and 2 X 10(6) viable cells/ml.

Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density)

Preservation: Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO

Storage temperature: liquid nitrogen vapor phase

Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2005

recommended serum:ATCC 30-2020

References: 49807: Baskar S, et al. Major histocompatibility complex class II+B7-1+ tumor cells are potent vaccines for stimulating tumor rejection in tumor-bearing mice. J. Exp. Med. 181: 619-629, 1995. PubMed: 7836917

53559: Baker P, et al. Mechanisms of tumor homograft rejection: The behavior of Sarcoma I ascites tumor in the A/Jax and the C57BL/6K mouse. Ann. N.Y. Acad. Sci. 101: 46-63, 1962.

53560: Dunham LJ, Stewart HL. SaI [Sa I, Sarcoma I]細(xì)胞A survey of transplantable and transmissible animal tumors. J. Natl. Cancer Inst. 13: 1299-1377, 1953. PubMed: 13035452