RAN-2細胞
數(shù)量： 大量
細胞形態(tài)： **樣
**類型： IgG2a
生長狀態(tài)： 懸浮生長
運輸方式： 凍存運輸
是否是腫瘤細胞： 0
物種來源： 小鼠
ATCC Number： TIB-119?
RAN-2細胞Designations: RAN-2 [alpha RAN-2, anti RAN-2]
Depositors: PF Bartlett, M Raff
Isotype: IgG2a
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: suspension
Organism: Mus musculus (B cell); Mus musculus (myeloma) deposited as mouse (B cell); mouse (myeloma)
Morphology: lymphoblast
Source: RAN-2細胞Cell Type: hybridoma: B lymphocyte;
Cellular Products: immunoglobulin; monoclonal antibody; against rat astrocyte; ependymal cell, retinal Muller cell and leptomeningeal cell (rat neural antigen-2, RAN-2)
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Comments: Animals were immunized with enriched preparations of rat astrocytes.
Spleen cells were fused with NS-1 myeloma cells.
RAN-2細胞The antibody does not react with cultured neurons, oligodendrocytes, Schwann cells, microglia or various non-neuronal cells.
Tested and found negative for ectromelia virus (mousepox).
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing: Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 x 10 exp5 cells/ml and maintain between 1 x 10 exp5 and 1 x 10 exp6 cells/ml.
References: 902: Bartlett PF, et al.RAN-2細胞 Rat neural antigen-2 (RAN-2): a cell surface antigen on astrocytes, ependymal cells, Muller cells and lepto-meninges defined by a monoclonal antibody. Brain Res. 204: 339-351, 1980. PubMed: 7006734
