Raszip 6細胞

是否是腫瘤細胞： 0

物種來源： 小鼠

器官來源： 胚胎

ATCC Number： CRL-1917?

運輸方式： 凍存運輸

細胞形態(tài)： 成纖維樣

生長狀態(tài)： 貼壁生長

品系： NIH/Swiss

年限： embryo

Raszip 6細胞數(shù)量： 大量

Designations: Raszip 6

Depositors: RA Weinberg

Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: adherent

Organism: Mus musculus

Morphology: fibroblast

Source: Organ: embryo

Strain: NIH/Swiss

Permits/Forms: Raszip 6細胞In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

Age: embryo

Comments: This line was established by G.P. Dotto, et al. in 1985 by transfecting psi2 cells with the raszip 6 DNA construct.

The cells produce a high titer of helper free retrovirus carrying the vHa-ras oncogene and the G418 resistance gene.

Propagation: ATCC complete growth medium: Dulbecco's modified Eagle's Medium with 4.5 g/L glucose and 0.5 mg/ml G418, 90%; fetal bovine serum, 10%

Subculturing: Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended

Medium Renewal: Raszip 6細胞Every 2 to 3 days

Remove spent medium, add fresh 0.25% trypsin, 0.03% EDTA solution, rinse and remove trypsin. Let the culture sit at room temperature (or at 37C) until the cells detach. Add fresh medium, aspirate and dispense into new flasks. Subculture every 6 to 8 days.

References: 22922: Dotto GP, et al. Specific growth response of ras-transformed embryo fibroblasts to tumour promoters. Nature 318: 472-475, 1985. PubMed: 4069218

26166: Mann R, et al. Construction of a retrovirus packaging mutant and its use to produce helper-free defective retrovirus. Cell 33: 153-159, 1983. Raszip 6細胞PubMed: 6678608