260-33C4細(xì)胞

細(xì)胞形態(tài)： **樣

**類型： IgM

數(shù)量： 大量

生長(zhǎng)狀態(tài)： 懸浮生長(zhǎng)

品系： 129GIX+

是否是腫瘤細(xì)胞： 0

物種來源： 小鼠

年限： *****

運(yùn)輸方式： 凍存運(yùn)輸

260-33C4細(xì)胞器官來源： 脾

ATCC Number： CRL-2667?

Designations: 260-33C4

Depositors: National Cancer Institute

Isotype: IgM

Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: suspension

Organism: Mus musculus (B cell); Mus musculus (myeloma) deposited as mouse (B cell); mouse (myeloma)

Morphology: lymphoblast

Source: Strain: 129GIX+

Organ: spleen

Cell Type: 260-33C4細(xì)胞hybridoma: B lymphocyte;

Cellular Products: immunoglobulin; monoclonal antibody; against a RAF 1 synthetic oncogene peptide

Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

Age: *****

Comments: Spleen cells were fused with Sp2/0 myeloma cells.

Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Temperature: 37.0°C

Subculturing: Protocol: 260-33C4細(xì)胞Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 to 2 X 10 exp5 viable cells/ml.Maintain cell density between 1 X 10 exp5 and 1 X 10 exp6 viable cells/ml.

Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density)

Preservation: Freeze medium: Complete growth medium 95%; DMSO, 5%

260-33C4細(xì)胞Storage temperature: liquid nitrogen vapor phase