20-10-5S細(xì)胞
ATCC Number： HB-23?
數(shù)量： 大量
生長(zhǎng)狀態(tài)： 懸浮生長(zhǎng)
細(xì)胞形態(tài)： **樣
**類(lèi)型： IgM; kappa light chain
是否是腫瘤細(xì)胞： 0
物種來(lái)源： 小鼠
運(yùn)輸方式： 凍存運(yùn)輸
Designations: 20-10-5S
20-10-5S細(xì)胞Depositors: DH Sachs, K Ozato
Isotype: IgM; kappa light chain
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: suspension
Organism: Mus musculus (B cell); Mus musculus (myeloma) deposited as mouse (B cell); mouse (myeloma)
Morphology: lymphoblast
Source: Cell Type: hybridoma: B lymphocyte;
Cellular Products: immunoglobulin; monoclonal antibody; against mouse T cell (mouse T lymphocyte); cytotoxic
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. 20-10-5S細(xì)胞Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Comments: Animals were immunized with spleen cells from C3H.SW mice.
Spleen cells were fused with Sp2/0-Ag14 myeloma cells.
The antibody appears to react with the Thy-1 antigen on T cells.
Tested and found negative for ectromelia virus (mousepox).
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC Hybri-Care Medium, Catalog No. 46-X. Hybri-Care Medium is supplied as a powder and should be reconstituted in 1 L cell-culture-grade water. To make the complete growth medium, add the following components to the base medium:
fetal bovine serum to a final concentration of 10%
1.5 g/L sodium bicarbonate for use with 5% CO2 in air atmosphere.
Subculturing: Medium Renewal: 20-10-5S細(xì)胞Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 46-X
20-10-5S細(xì)胞References: 938: Auchincloss H Jr., et al. A monoclonal antibody detecting unusual Thy-1 determinants. J. Immunol. 128: 1584-1589, 1982. PubMed: 6174608
