UV135細(xì)胞
是否是腫瘤細(xì)胞： 0
物種來源： 倉鼠
器官來源： 卵巢
運(yùn)輸方式： 凍存運(yùn)輸
ATCC Number： CRL-1867?
細(xì)胞形態(tài)： 上皮樣
生長狀態(tài)： 單層、懸浮
數(shù)量： 大量
Designations: UV135 (UV sensitive mutant of CHO)
Depositors: LH Thompson
Biosafety Level: 1
UV135細(xì)胞Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: monolayer and suspension
Organism: Cricetulus griseus
Morphology: epithelial-like
Source: Organ: ovary
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Gender: female
Comments: This line is a derivative of the CHO-K1 cell line (see ATCC CCL-61).
UV135 is a UV sensitive line derived from AA8 (see ATCC CRL-1859).
The line is defective in nucleotide excision repair, is sensitive to bulky adduct mutagens and belongs to excision repair complementation group 5.
Propagation: UV135細(xì)胞ATCC complete growth medium: Alpha minimum essential medium without ribonucleosides and deoxyribonucleosides, 90%; fetal bovine serum, 10%
Subculturing: Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:12 is recommended
Medium Renewal: 2 to 3 times per week
To subculture attached cells, remove the medium and rinse the cells with fresh 0.25% trypsin, 0.03% EDTA.
Let the culture sit at 37C until the cells detach (about 5 minutes).
Add fresh medium, aspirate and transfer to new flasks.
The suspended cells are viable and can be used to start new cultures.
Doubling Time: 13 hrs
References: 1767: Thompson LH, et al. Repair of DNA adducts in asynchronous CHO cells and the role of repair in cell killing and mutation induction in synchronous cells treated with 7-bromomethylbenz[a]anthracene. Somatic Cell Mol. Genet. 10: 183-194, 1984. PubMed: 6584989
1768: Thompson LH, et al. Genetic diversity of UV-sensitive DNA repair mutants of Chinese hamster ovary cells. Proc. Natl. Acad. Sci. USA 78: 3734-3737, 1981. PubMed: 6943579
1769: Hoy CA, et al. Defective DNA cross-link removal in Chinese hamster cell mutants hypersensitive to bifunctional alkylating agents. Cancer Res. 45: 1737-1743, 1985. PubMed: 3919945
1770: Busch D, et al.UV135細(xì)胞 Summary of complementation groups of UV-sensitive CHO cell mutants isolated by large-scale screening. Mutagenesis 4: 349-354, 1989. PubMed: 2687628
21855: . Cellular responses to DNA damage. New York: Liss; 1983.
32479: Bessho T, et al. Initiation of DNA interstrand cross-link repair in humans: the nucleotide excision repair system makes dual incisions 5" to the cross-linked base and removes a 22- to 28-nucleotide-long damage-free strand. Mol. Cell. Biol. 17: 6822-6830, 1997. PubMed: 9372913
32924: Reardon JT, et al. Isolation and characterization of two human transcription factor IIH (TFIIH)-related complexes: ERCC2/CAKand TFIIH. Proc. Natl. Acad. Sci. USA 93: 6482-6487, 1996. PubMed: 8692841
58395: Thompson LH, et al. UV135細(xì)胞Hypersensitivity to mutation and sister-chromatid-exchange induction in CHO cell mutants defective in incising DNA containing UV lesions. Somatic Cell Genet. 8: 759-773, 1982. PubMed: 7163954
