USASK/DSIL-LHRH-A1細(xì)胞

ATCC Number： HB-9094?

生長狀態(tài)： 懸浮生長

運(yùn)輸方式： 凍存運(yùn)輸

數(shù)量： 大量

細(xì)胞形態(tài)： **樣

**類型： IgG1

是否是腫瘤細(xì)胞： 0

物種來源： 小鼠

USASK/DSIL-LHRH-A1細(xì)胞Designations: USASK/DSIL-LHRH-A1

Depositors: University of Saskatchewan

Isotype: IgG1

Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: suspension

Organism: Mus musculus (B cell); Mus musculus (myeloma) deposited as mouse (B cell); mouse (myeloma)

Morphology: lymphoblast

Source: Cell Type: USASK/DSIL-LHRH-A1細(xì)胞hybridoma: B lymphocyte;

Cellular Products: immunoglobulin; monoclonal antibody; against the carboxy terminal end of gonadotropin releasing hormone (also called luteinizing hormone releasing hormone (LHRH)

Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

Tumorigenic: Yes

Comments: USASK/DSIL-LHRH-A1細(xì)胞Animals were immunized with LHRH conjugated to bovine serum albumin.

Spleen cells were fused with P3-NSI/1-Ag4-1 myeloma cells.

A feeder layer is required to recover the cells from a frozen ampule. Cells are grown at ATCC on 56-X feeder layer, irradiated STO cells. It is recommended that the feeder cells be plated 24 hours before use at 2 X 10(6)/T75 in order to obtain a 30% confluent monolayer.

Propagation: ATCC complete growth medium: RPMI 1640 medium with 2 mM L-glutamine and 50 mg/l gentamicin, 70%; NCTC 109 medium, 10%; fetal bovine serum, 20%

Subculturing: Medium Renewal: Every 2 to 3 days

Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml. USASK/DSIL-LHRH-A1細(xì)胞Cells are grown at ATCC on 56-X feeder layer, irradiated STO cells. It is recommended that the feeder cells be plated 24 hours before use at 2 X 10(6)/T75 in order to obtain a 30% confluent monolayer.

Preservation: Culture medium, 95%; DMSO, 5%

References: 58144: Silversides DW, et al. Monoclonal antibodies against LHRH: development and immunoactivity in vivo and in vitro. J. Reprod. Immunol. 7: 171-184, 1985. PubMed: 3884802