S6F1細(xì)胞

生長(zhǎng)狀態(tài)： 懸浮生長(zhǎng)

細(xì)胞形態(tài)： **樣

**類型： IgG1

是否是腫瘤細(xì)胞： 0

物種來源： 小鼠

ATCC Number： HB-9579?

運(yùn)輸方式： 凍存運(yùn)輸

數(shù)量： 大量

S6F1細(xì)胞Designations: S6F1

Depositors: Dana Farber Cancer Inst.

Isotype: IgG1

Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: suspension

Organism: Mus musculus (B cell); Mus musculus (myeloma) deposited as mouse (B cell); mouse (myeloma)

Morphology:S6F1細(xì)胞 lymphoblast

Source: Cell Type: hybridoma: B lymphocyte;

Cellular Products: immunoglobulin; monoclonal antibody; against a subset of human CD8 (LFA-1) positive T cells

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Comments: Animals were immunized with the 1670 cell line produced by immortalizing spleen cells from a whitelip tamarin with Herpesvirus saimiri.

Spleen cells were fused with P3/NSI/1-Ag4-1 myeloma cells.

S6F1細(xì)胞The antibody recognizes a subset of CD8 positive T cells that have cytotoxic effector activity.

The S6F1 epitope is absent on CD8 positive suppressor cells.

Propagation: ATCC complete growth medium: Dulbecco's modified Eagle's medium with 4.5 g/L glucose, 2 mM L-glutamine, 0.1 mM hypoxanthine, and 0.032 mM thymidine, 80%; heat-inactivated fetal bovine serum, 10%; heat-inactivated horse serum, 10%

Subculturing: Medium Renewal: Every 2 to 3 days

Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 2 X 10 exp5 and 1 X 10 exp6 cells/ml.

References: 21964: S6F1細(xì)胞Schlossman SF, Morimoto C. Monoclonal antibody specific to a novel epitope of the LFA-1 antigen of human T lymphocytes. US Patent 5,002,869 dated Mar 26 1991