200-3-G6-4 (20.4)細(xì)胞
ATCC Number： HB-8737?
數(shù)量： 大量
運(yùn)輸方式： 凍存運(yùn)輸
生長狀態(tài)： 懸浮生長
是否是腫瘤細(xì)胞： 0
物種來源： 小鼠
細(xì)胞形態(tài)： **樣
**類型： IgG1
200-3-G6-4 (20.4)細(xì)胞Designations: 200-3-G6-4 (20.4)
Depositors: Wistar Institute
Isotype: IgG1
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: suspension
Organism: Mus musculus (B cell); Mus musculus (myeloma) deposited as mouse (B cell); mouse (myeloma)
Morphology: lymphoblast
Source: Cell Type: hybridoma: B lymphocyte;
Cellular Products: 200-3-G6-4 (20.4)細(xì)胞immunoglobulin; monoclonal antibody; against nerve growth factor (NGF) receptor of primates
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Tumorigenic: Yes
Comments: Animals were immunized with human melanoma cell lines.
Spleen cells were fused with P3X63Ag8 myeloma cells.
The antibody binds to the 75000 dalton NGF receptor of primates.
Propagation: ATCC complete growth medium: Iscove's modified Dulbecco's medium with 2 mM L-glutamine, 90%; fetal bovine serum, 10%
Temperature: 37.0°C
Subculturing: Protocol: Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 1 X 10(5) cells/ml and maintain between 1 X 10(5) and 1 X 10(6) cells/ml.
Medium Renewal: every 2 to 3 days
Preservation: 200-3-G6-4 (20.4)細(xì)胞Freeze medium: Culture medium, 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor phase
Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2005
References: 3939: Koprowski H, et al. Diagnostic method for detection of neural crest disease. US Patent 4,786,593 dated Nov 22 1988
3940: Johnson EM Jr.. Tumor diagnostic method. US Patent 4,855,241 dated Aug 8 1989
23378: Levi AD, et al. The functional characteristics of Schwann cells cultured from human peripheral nerve after transplantation into a gap within the rat sciatic nerve. J. Neurosci. 14: 1309-1319, 1994. 200-3-G6-4 (20.4)細(xì)胞PubMed: 8120626
