S49.1TB.2細胞

生長狀態(tài)： 懸浮生長

細胞類型： T**細胞

是否是腫瘤細胞： 0

物種來源： 小鼠

ATCC Number： TIB-30?

相關(guān)**： **瘤

運輸方式： 凍存運輸

品系： BALB/c

細胞形態(tài)： **樣

S49.1TB.2細胞數(shù)量： 大量

Designations: S49.1TB.2

Depositors: A Harris

Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: suspension

Organism: Mus musculus

Morphology: lymphoblast

Source: Disease: lymphoma

Strain: BALB/c

Cell Type: S49.1TB.2細胞T lymphocyte;

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Antigen Expression: H-2d; Thy-1.2; TL

Comments: This line was derived from S49.1 (ATCC TIB-28) by selecting for resistance to 10 mM thymidine and 0.1 mM 5-bromo-2'-deoxyuridine.

Like the parent line, the cells are sensitive to 0.001 mM cortisol (hydrocortisone), 5 mcg/ml phytohemagglutinin and 0.01 mM 6-thioguanine.

Tested and found negative for ectromelia virus (mousepox).

Propagation: ATCC complete growth medium: Dulbecco's modified Eagle's medium, 90%; horse serum, 10%

Subculturing: S49.1TB.2細胞Medium Renewal: Every 2 to 3 days

Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.

References: 1130: Ralph P, et al. Independence of theta and TL surface antigens and killing by thymidine, cortisol, phytohemagglutinin, and cyclic AMP in a murine lymphoma. Biochem. Biophys. Res. Commun. 55: 1085-1091, 1973. PubMed: 4358928

22154: Horibata K, Harris AW. Mouse myelomas and lymphomas in culture. Exp. Cell Res. 60: 61-77, 1970. PubMed: 5439579

22241: Schiller JG, et al. S49.1TB.2細胞Glucocorticoid receptors in lymphoma cells in culture: relationship to glucocorticoid killing activity. Science 171: 189-191, 1971. PubMed: 4395230