RA3-3A1/6.1細胞

生長狀態(tài)： 懸浮生長

細胞形態(tài)： **樣

**類型： IgM

運輸方式： 凍存運輸

是否是腫瘤細胞： 0

物種來源： 大鼠

ATCC Number： TIB-146?

數(shù)量： 大量

RA3-3A1/6.1細胞Designations: RA3-3A1/6.1

Depositors: RL Coffman

Isotype: IgM

Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: suspension

Organism: Rattus norvegicus (B cell); Mus musculus (myeloma) deposited as rat (B cell); mouse (myeloma)

Morphology: lymphoblast

Source: RA3-3A1/6.1細胞Cell Type: hybridoma: B lymphocyte;

Cellular Products: immunoglobulin; monoclonal antibody; against the B220 mouse B cell antigen (200000 dalton form of the T200 glycoprotein, CD45R)

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Comments: Animals were immunized with the mouse cell line RAW 112.

Spleen cells were fused with the S194/5.XX0.BU.1 myeloma.

Tested and found negative for ectromelia virus (mousepox).

Propagation: RA3-3A1/6.1細胞ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Subculturing: Medium Renewal: Every 2 to 3 days

Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 3 x 10 exp4 cells/ml, and do not allow the cell concentration to exceed 5 x 10 exp5 cells/ml.

References: 925: Coffman RL, Weissman IL. A monoclonal antibody that recognizes B cells and B cell precursors in mice. J. Exp. Med. 153: 269-279, 1981. PubMed: 6787164

927: Coffman RL, Weissman IL. RA3-3A1/6.1細胞B220: a B cell-specific member of the T200 glycoprotein family. Nature 289: 681-683, 1981. PubMed: 6970340

32948: Wong P, Rudensky AY. Phenotype and function of CD4+ T cells in mice lacking invariant chain. J. Immunol. 156: 2133-2142, 1996. PubMed: 8690902