S49 (Thy-1-a)細(xì)胞

細(xì)胞類(lèi)型： T**細(xì)胞

是否是腫瘤細(xì)胞： 0

物種來(lái)源： 小鼠

生長(zhǎng)狀態(tài)： 懸浮生長(zhǎng)

運(yùn)輸方式： 凍存運(yùn)輸

細(xì)胞形態(tài)： **樣

品系： BALB/c

數(shù)量： 大量

ATCC Number： TIB-36?

S49 (Thy-1-a)細(xì)胞相關(guān)**： **瘤

Designations: S49 (Thy-1-a)

Depositors: R Hyman

Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: suspension

Organism: Mus musculus

Morphology: lymphoblast

Source: Disease: lymphoma

Strain: BALB/c

Cell Type: T lymphocyte;

Permits/Forms: S49 (Thy-1-a)細(xì)胞In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

Applications: transfection host

Antigen Expression: H-2d; TL

Comments: This variant of S49.1TB.2 (ATCC TIB-30) does not express Thy-1.2 antigen.

The line was selected by 5 cycles of immunoselection using anti Thy-1 antiserum and complement.

This cell line is a Class A Thy-1- mutant.

The defect affects the biosysnthesis and/or assembly of the glycophospholipid membrane anchor.

Tested and found negative for ectromelia virus (mousepox).

Propagation: ATCC complete growth medium: Dulbecco's modified Eagle's medium, 90%; horse serum, 10%

Subculturing: S49 (Thy-1-a)細(xì)胞Medium Renewal: Every 2 to 3 days

Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.

References: 1129: Hyman R. Studies on surface antigen variants. Isolation of two complementary variants for Thy 1.2. J. Natl. Cancer Inst. 50: 415-422, 1973. PubMed: 4702114

1130: Ralph P, et al. Independence of theta and TL surface antigens and killing by thymidine, cortisol, phytohemagglutinin, and cyclic AMP in a murine lymphoma. Biochem. Biophys. Res. Commun. 55: 1085-1091, 1973. PubMed: 4358928

22154: Horibata K, Harris AW. Mouse myelomas and lymphomas in culture. Exp. Cell Res. 60: 61-77, 1970. PubMed: 5439579

22304: Trowbridge IS, et al. The synthesis and properties of T25 blycoprotein in Thy-1-negative mutant lymphoma cells. Cell 14: 21-32, 1978. PubMed: 667934

22768: Conzelmann A, et al. Anchoring of membrane proteins via phosphatidylinositol is deficient in two classes of Thy-1 negative mutant lymphoma cells. EMBO J. 5: 3291-3296, 1986. S49 (Thy-1-a)細(xì)胞PubMed: 2880714