R9ab細(xì)胞
運(yùn)輸方式： 凍存運(yùn)輸
細(xì)胞形態(tài)： 成纖維樣
細(xì)胞類型： 成纖維細(xì)胞
是否是腫瘤細(xì)胞： 0
物種來源： 其他
數(shù)量： 大量
年限： fetus
生長狀態(tài)： 貼壁生長
ATCC Number： CCL-193?
R9ab細(xì)胞器官來源： 肺
相關(guān)**： 正常
Designations: R9ab
Depositors: EM Earley
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: adherent
Organism: Oryctolagus cuniculus
Morphology: fibroblast
Source: Organ: lung
Disease: normal
Cell Type: R9ab細(xì)胞fibroblast
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Cytogenetic Analysis: normal; diploid; stable
Age: fetus
Gender: female
Comments: R9ab was derived from explants of biopsy material. Subsequent cell outgrowths were trypsinized and the resulting cell suspension was used to initiate the line.
The donor indicated that the life expectancy of this line is at least 60 passages.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Temperature: 37.0°C
Subculturing:R9ab細(xì)胞 Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:3 is recommended
Medium Renewal: Every 2 to 3 days
Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach.
Add fresh culture medium, aspirate and dispense into new culture flasks.
Preservation: culture medium 95%; DMSO, 5%
Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2003
recommended serum:ATCC 30-2020
References: 32878: Castagliuolo I, et al. R9ab細(xì)胞Saccharomyces boulardii protease inhibits Clostridium difficile toxin A effects in the rat ileum. Infect. Immun. 64: 5225-5232, 1996. PubMed: 8945570
