2.28 M1 [A2,28M1]細(xì)胞

運(yùn)輸方式： 凍存運(yùn)輸

生長(zhǎng)狀態(tài)： 懸浮生長(zhǎng)

ATCC Number： HB-166?

細(xì)胞形態(tài)： **樣

**類(lèi)型： IgM

數(shù)量： 大量

是否是腫瘤細(xì)胞： 0

物種來(lái)源： 小鼠

2.28 M1 [A2,28M1]細(xì)胞Designations: 2.28 M1 [A2,28M1]

Depositors: F Grumet

Isotype: IgM

Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: suspension

Organism: Mus musculus (B cell); Mus musculus (myeloma) deposited as mouse (B cell); mouse (myeloma)

Morphology: lymphoblast

Source: Cell Type: hybridoma: B lymphocyte;

Cellular Products:2.28 M1 [A2,28M1]細(xì)胞 immunoglobulin; monoclonal antibody; against HLA A2, A28; cytotoxic

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Comments: Animals were immunized with human peripheral blood leukocytes (HLA phenotype A2,Bw6,Bw48,Bw60,Cw4).

Spleen and lymph node cells were fused with Sp2/08A2 myeloma cells.

The antibody is cytotoxic for a subpopulation of HLA A2 positive cells and HLA A28 positive cells at an appropriate titer.

The antibody recognizes an epitope at the amino terminal end of the alpha 2 domain of the HLA-A2 molecule. [39936]

Tested and found negative for ectromelia virus (mousepox).

Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC Hybri-Care Medium, Catalog No. 46-X. Hybri-Care Medium is supplied as a powder and should be reconstituted in 1 L cell-culture-grade water. To make the complete growth medium, add the following components to the base medium:

fetal bovine serum to a final concentration of 10%

2.28 M1 [A2,28M1]細(xì)胞1.5 g/L sodium bicarbonate for use with 5% CO2 in air atmosphere

0.05 mM 2-Mercaptoethanol

Temperature: 37.0°C

Subculturing: Medium Renewal: Every 2 to 3 days

Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 5 X 10 exp4 cells/ml and maintain between 5 X 10 exp4 and 5 X 10 exp5 cells/ml.

Preservation: culture medium 95%; DMSO, 5%

Related Products: recommended serum:ATCC 30-2020

References: 39936: Hogan KT, Brown SL. Localization and characterization of serologic epitopes on HLA-A2. 2.28 M1 [A2,28M1]細(xì)胞Hum. Immunol. 33: 185-192, 1992. PubMed: 1377666