UCD/AB 6.01細(xì)胞

是否是腫瘤細(xì)胞： 0

物種來(lái)源： 小鼠

生長(zhǎng)狀態(tài)： 懸浮生長(zhǎng)

運(yùn)輸方式： 凍存運(yùn)輸

細(xì)胞形態(tài)： **樣

ATCC Number： HB-8693?

數(shù)量： 大量

Designations: UCD/AB 6.01

Depositors: Regents of the University of California

UCD/AB 6.01細(xì)胞Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: suspension

Organism: Mus musculus (B cell); Mus musculus (myeloma) deposited as mouse (B cell); mouse (myeloma)

Morphology: lymphoblast

Source: Cell Type: hybridoma: B lymphocyte;

Cellular Products: immunoglobulin; monoclonal antibody; against a human mammary carcinoma cell line (MCF7, see ATCC HTB-22)

Permits/Forms: UCD/AB 6.01細(xì)胞In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

Comments: Animals were immunized with the MCF7 human mammary cancer cell line.

Spleen cells were fused with P3X63Ag8.653 myeloma cells.

The antibody is specific for type 8 cytokeratin (52000 dalton).

It cross-reacts somewhat with types 18 and 19 cytokeratins (46000 dalton and 40000 dalton, respectively).

UCD/AB 6.01細(xì)胞The antibody can be used to detect cytokeratin released by neoplastic cells into serum.

Propagation: ATCC complete growth medium: RPMI 1640 medium with non-essential amino acids, 90%; fetal bovine serum, 10%

Subculturing: Medium Renewal: Every 2 to 3 days

Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.

Preservation: culture medium, 90%; DMSO, 10%

References: 3931: Cardiff RD, et al. Cytokeratin tumor markers and assays for their detection. UCD/AB 6.01細(xì)胞US Patent 4,775,620 dated Oct 4 1988