R6.5.D6.E9.B2 [R6-5-D6]細胞

器官來源： 脾

生長狀態(tài)： 懸浮生長

ATCC Number： HB-9580?

是否是腫瘤細胞： 0

物種來源： 小鼠

細胞形態(tài)： **樣

**類型： mouse IgG2a kappa

年限： *****

運輸方式： 凍存運輸

R6.5.D6.E9.B2 [R6-5-D6]細胞品系： BALB/c

數(shù)量： 大量

Designations: R6.5.D6.E9.B2 [R6-5-D6]

Depositors: Dana Farber Cancer Inst.

Isotype: mouse IgG2a kappa

Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: suspension

Organism: Mus musculus (B cell); Mus musculus (myeloma) deposited as mouse (B cell); mouse (myeloma)

Morphology: lymphoblast

Source: Organ: spleen

Strain: R6.5.D6.E9.B2 [R6-5-D6]細胞BALB/c

Cell Type: hybridoma: B lymphocyte;

Cellular Products: immunoglobulin; monoclonal antibody; against human intracellular adhesion molecule 1 (ICAM-1)

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Age: *****

Comments: Animals were immunized with the EBV transformed lymphoblast cell line, JY.

Spleen cells were fused with P3X63Ag8.653 myeloma cells.

The antibody binds to ICAM-1 and inhibits the interaction of ICAM-1 with leukocytes.

Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Atmosphere: air, 95%; carbon dioxide (CO2), 5%

R6.5.D6.E9.B2 [R6-5-D6]細胞Temperature: 37.0°C

Subculturing: Protocol: Cultures can be maintained by addition of fresh medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 2 X 10(4) viable cells/ml. Do not allow the cell concentration to reach 6 X 10(5) cells/ml.

Interval: Maintain cultures at a cell concentration between 3 X 10(4) and 5 X 10(5) cells/ml.

Medium Renewal: Every 2 to 3 days

Preservation: Freeze medium: Complete growth medium supplemented with extra 10% FBS and 7.5% (v/v) DMSO

Storage temperature: liquid nitrogen vapor phase

Doubling Time: 12 hours

Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2001

recommended serum:ATCC 30-2020

References: 22051: Springer TA, et al. R6.5.D6.E9.B2 [R6-5-D6]細胞Intercellular adhesion molecules, and their binding ligands. US Patent 5,284,931 dated Feb 8 1994