S3D3 [3D3b]細胞

生長狀態(tài)： 懸浮生長

是否是腫瘤細胞： 0

物種來源： 小鼠

ATCC Number： HB-8331?

細胞形態(tài)： **樣

**類型： IgG2a

數(shù)量： 大量

運輸方式： 凍存運輸

S3D3 [3D3b]細胞Designations: S3D3 [3D3b]

Depositors: American Cyanamid Co.

Isotype: IgG2a

Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: suspension

Organism: Mus musculus (B cell); Mus musculus (myeloma) deposited as mouse (B cell); mouse (myeloma)

Morphology: lymphoblast

Source: Cell Type: hybridoma: B lymphocyte;

Cellular Products: S3D3 [3D3b]細胞immunoglobulin; monoclonal antibody; against Eimeria tenella sporozoites

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Comments: Animals were immunized with E. tenella sporozoites.

Spleen cells were fused with P3-NSI/1-Ag4-1 myeloma cells.

Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Iscove's Modified Dulbecco's Medium, Catalog No. 30-2005. S3D3 [3D3b]細胞To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Temperature: 37.0°C

Subculturing: Medium Renewal: Every 2 to 3 days

Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.

Preservation: culture medium 95%; DMSO, 5%

Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2005

recommended serum:ATCC 30-2020

References: 3885: Wong RB, et al. S3D3 [3D3b]細胞Antigens and monoclonal antibodies reactive against sporozoites of Eimeria spp.. US Patent 4,710,377 dated Dec 1 1987