UC8-1B9細胞

生長狀態(tài)： 懸浮生長

數(shù)量： 大量

是否是腫瘤細胞： 0

物種來源： 倉鼠

細胞形態(tài)： **樣

**類型： IgG

運輸方式： 凍存運輸

ATCC Number： CRL-1968?

Designations: UC8-1B9

UC8-1B9細胞Depositors: JA Bluestone

Isotype: IgG

Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: suspension

Organism: UC8-1B9細胞Cricetulus migratorius (B cell); Mus musculus (myeloma) deposited as hamster, Armenian (B cell); mouse (myeloma)

Morphology: lymphoblast

Source: Cell Type: hybridoma: B lymphocyte;

Cellular Products: immunoglobulin; monoclonal antibody; against dinitrophenyl (DNP)

Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

UC8-1B9細胞Comments: Animals were immunized with DNP derivatized KLH (keyhole limpet hemocyanin).

Spleen cells were fused with Sp2/0-Ag14 myeloma cells.

The antibody is useful as a control monoclonal antibody when using other hamster derived hybridomas.

Propagation: ATCC complete growth medium: Iscove's modified Dulbecco's medium, 90%; fetal bovine serum, 10%

Subculturing: Medium Renewal: Every 2 to 3 days

Cultures can be maintained by addition or replacement of fresh medium. UC8-1B9細胞Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.