20H11細(xì)胞

運(yùn)輸方式： 凍存運(yùn)輸

器官來(lái)源： 脾

數(shù)量： 大量

細(xì)胞形態(tài)： **樣

ATCC Number： CRL-9300?

細(xì)胞類(lèi)型： B**細(xì)胞

是否是腫瘤細(xì)胞： 0

物種來(lái)源： 人

生長(zhǎng)狀態(tài)： 懸浮生長(zhǎng)

Designations: 20H11

20H11細(xì)胞Depositors: Genetic Systems Corp.

Biosafety Level: 2 [Cells Contain HERPESVIRUS ]

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: suspension

Organism: Homo sapiens

Morphology: lymphoblast

Source: Organ: spleen

Cell Type: B lymphocyte; Epstein-Barr virus (EBV) transformed

Cellular Products: immunoglobulin; monoclonal antibody; against Pseudomonas aeruginosa (flagella type b)

Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. 20H11細(xì)胞Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

Comments: Peripheral blood B cells from a patient immunized with high molecular weight polysaccharide from P. aeruginosa were transformed with Epstein-Barr virus.

The antibody reacts with P aeruginosa Fisher immunotypes 2, 6 and 7.

The antibody cross-reacts extensively with clinical isolates of P. aeruginosa bearng type b flagella.

Propagation: ATCC complete growth medium: 20H11細(xì)胞Iscove's modified Dulbecco's medium with 2 mM L-glutamine, 85%; heat-inactivated fetal bovine serum, 15%

Subculturing: Medium Renewal: Every 3 to 4 days

Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.

Doubling Time: 24 to 36 hrs

References: 3871: Rosok MJ, Lostrom ME. 20H11細(xì)胞Monoclonal antibodies to Pseudomonas aeruginosa flagella. US Patent 4,834,976 dated May 30 1989