UC3-10A6細(xì)胞
數(shù)量： 大量
生長狀態(tài)： 懸浮生長
是否是腫瘤細(xì)胞： 0
物種來源： 倉鼠
細(xì)胞形態(tài)： **樣
**類型： IgG
運(yùn)輸方式： 凍存運(yùn)輸
ATCC Number： CRL-1988?
Designations: UC3-10A6
UC3-10A6細(xì)胞Depositors: JA Bluestone
Isotype: IgG
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: suspension
Organism: Cricetulus migratorius (B cell); Mus musculus (myeloma) deposited as hamster, Armenian (B cell); mouse (myeloma)
Morphology: lymphoblast
Source: Cell Type: hybridoma: B lymphocyte;
Cellular Products: immunoglobulin; monoclonal antibody; against mouse gamma/delta T cell receptor (TCR)
Permits/Forms: UC3-10A6細(xì)胞In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Applications: The antibody reacts with the murine T cell receptor gamma/delta TCR.
The antibody is specific for receptors containing the V gamma 2 segment.
Comments: Animals were immunized with the G8 mouse T cell clone (TCR gamma/ delta).
Spleen cells were fused with Sp2/0-Ag14 myeloma cells.
UC3-10A6細(xì)胞The antibody reacts with the murine T cell receptor gamma/delta TCR.
The antibody is specific for receptors containing the V gamma 2 segment.
Propagation: ATCC complete growth medium: Iscove's modified Dulbecco's medium, 90%; fetal bovine serum, 10%
Subculturing: Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
References: 22933: Dent AL, et al. UC3-10A6細(xì)胞Self-reactive gamma delta T cells are eliminated in the thymus. Nature 343: 714-719, 1990. PubMed: 1968227
