S194/5.XXO-1細胞
品系： BALB/c
ATCC Number： TIB-19?
運輸方式： 凍存運輸
相關**： 多發(fā)性骨髓瘤
數(shù)量： 大量
生長狀態(tài)： 懸浮生長
細胞形態(tài)： **樣
**類型： IgA (not secreted); kappa light chain (not secreted)
細胞類型： B**細胞
是否是腫瘤細胞： 0
S194/5.XXO-1細胞物種來源： 小鼠
Designations: S194/5.XXO-1
Depositors: R Hyman, P Ralph, S Sarkar
Isotype: IgA (not secreted); kappa light chain (not secreted)
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: suspension
Organism: S194/5.XXO-1細胞Mus musculus
Morphology: lymphoblast
Source: Disease: plasmacytoma; myeloma
Strain: BALB/c
Cell Type: B lymphocyte;
Cellular Products: immunoglobulin; monoclonal antibody; not secreted
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Antigen Expression: H-2d; PC.1
Comments: This line was derived from an IgA secreting mineral oil induced BALB/c myeloma.
Both heavy and light chains are synthesized but not secreted.
Tested and found negative for ectromelia virus (mousepox).
Propagation: S194/5.XXO-1細胞ATCC complete growth medium: Dulbecco's modified Eagle's medium, 90%; horse serum, 10%
Subculturing: Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
References: 22154: Horibata K, Harris AW. Mouse myelomas and lymphomas in culture. Exp. Cell Res. 60: 61-77, 1970. PubMed: 5439579
22522: Hyman R, et al. Cell-specific antigens and immunoglobulin synthesis of murine myeloma cells and their variants. J. Natl. Cancer Inst. 48: 173-184, 1972. PubMed: 4119881
33006: Genuario RR, Perry RP. The GA-binding protein can serve as both an activator and repressor of ribosomal protein gene transcription. J. Biol. Chem. 271: 4388-4395, 1996. S194/5.XXO-1細胞PubMed: 8626789
