R2-9A5 [R2-9A5.2A2.1A10]細(xì)胞

生長(zhǎng)狀態(tài)： 懸浮生長(zhǎng)

運(yùn)輸方式： 凍存運(yùn)輸

數(shù)量： 大量

**類型： IgG2b

是否是腫瘤細(xì)胞： 0

物種來(lái)源： 褐鼠

ATCC Number： CRL-2357?

Designations: R2-9A5 [R2-9A5.2A2.1A10]

R2-9A5 [R2-9A5.2A2.1A10]細(xì)胞Depositors: DH Presky

Isotype: IgG2b

Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: suspension

Organism: Rattus norvegicus (B cell); Mus musculus (myeloma) deposited as rat

Morphology:

Source: Cell Type: hybridoma: B lymphocyte;

Cellular Products: R2-9A5 [R2-9A5.2A2.1A10]細(xì)胞immunoglobulin; monoclonal antibody; against mouse interleukin 12 (IL-12) p75

Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

Comments: The hybridoma cell line R2-9A5 [R2-9A5.2A2.1A10] was established by David Presky and Victoria Wilkinson in 1993 and subcloned twice.

The antibody is a useful tool for detection of mouse IL-12p75 by ELISA and Western Blot analysis.

R2-9A5 was formed by the fusion of SP2/0 mouse myeloma cells with splenocytes from an ***** female Lewis rat inoculated with recombinant mouse IL-12 p75.

Propagation: R2-9A5 [R2-9A5.2A2.1A10]細(xì)胞ATCC complete growth medium: Iscove's Modified Dulbecco's Medium with 4 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate supplemented with 0.05 mM 2-mercaptoethanol and 100 U/ml recombinant human IL-6, 90%; fetal bovine serum, 10%.

Temperature: 37.0°C

Subculturing: Medium Renewal: Every 2 to 3 days

Cultures can be maintained by addition or replacement of fresh medium. Alternately, cultures can be established by centrifugation with subsequent resuspension in fresh culture medium.

Establish new cultures at 2 X 10 exp5 viable cells/ml and maintain at between 1 X 10 exp5 and 1 X 10 exp6 viable cells/ml.

Preservation: Culture medium, 95%; DMSO, 5%

References: 34475: Wilkinson VL, et al. Characterization of anti-mouse IL-12 monoclonal antibodies and measurement of mouse IL-12 by ELISA. J. Immunol. Methods 189: 15-24, 1996. R2-9A5 [R2-9A5.2A2.1A10]細(xì)胞PubMed: 8576576